Advertisement
Research Article| Volume 76, ISSUE 4, P652-658, October 1970

Download started.

Ok

Effect of endotoxin on normal and 5-fluorouracil-suppressed hematopoietic stem cells

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Endotoxin (S. typhosa) injected into mice 4 hours after injection of 150 mg. per kilogram of 5-fluorouracil (5-FU) enhanced their hematopoietic recovery. The accelerated regeneration after 5-FU was first demonstrable on Day 2 in the number of colony-forming units per femur, on Day 4 in the total nucleated marrow count, and on Day 6 in peripheral blood counts. Endotoxin injected 24 hours before 5-fluorouracil had the opposite effect. It delayed demonstrable regeneration of colony-forming units by 4 days and caused a severe and long-lasting granulocytopenia. It is concluded that endotoxin, either directly or indirectly, triggers normally resting colony-forming units into proliferative cycles.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Smith W.W.
        • Alderman I.M.
        • Gillespie R.E.
        Resistance to experimental infection and mobilization of granulocytes in irradiated mice treated with bacterial endotoxin.
        Amer. J. Physiol. 1958; 192: 263
        • Smith W.W.
        • Brecher G.
        • Fred S.
        • Budd R.A.
        Effect of endotoxin on the kinetics of hemopoietic colony-forming cells in irradiated mice.
        Radiat. Res. 1966; 27: 710
        • Hanks G.E.
        • Ainsworth E.J.
        Endotoxin protection and colony-forming units.
        Radiat. Res. 1964; 32: 367
        • Gidali J.
        • Feher I.
        • Gallyas A.
        • Varteresz V.
        Colony-forming cells in irradiated, partially shielded, endotoxin-treated mice.
        Radiat. Res. 1969; 39: 305
        • Hanna Jr., M.G.
        • Nettersheim P.
        • Fisher W.D.
        • Peters L.C.
        • Francis M.W.
        Serum alpha globulin fraction: Survival and recovery effect in irradiated mice.
        Science. 1967; 157: 1458
        • Berenblum I.
        • Burger M.
        • Knyszynski A.
        Regeneration of bone marrow cells and thymus induced by 19s alpha-2 globulin in irradiated mice.
        Nature. 1968; 217: 857
        • Reissmann K.R.
        • Samorapoompichit S.
        Effect of erythropoietin on regeneration of hematopoietic stem cells after 5-fluorouracil administration.
        J. Lab. Clin. Med. 1969; 73: 544
        • Bruce W.R.
        • Meeker B.E.
        Comparison of the sensitivity of hematopoietic colony-forming cells in different proliferative states to 5-fluorouracil.
        J. Nat. Cancer Inst. 1967; 38: 401
        • McCulloch E.A.
        • Till J.E.
        The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative transplantation into irradiated mice.
        Radiat. Res. 1960; 13: 115
        • Gagon T.E.
        • Athens J.W.
        • Boggs D.R.
        • Cartwright G.E.
        An evaluation of the variance of leukocyte counts as performed with the Hemocytometer, Coulter, and Fisher instruments.
        Amer. J. Clin. Path. 1966; 36: 684
        • Bruce W.R.
        • McCulloch E.A.
        The effect of erythropoietic stimulation on hemopoietic colony-forming cells of mice.
        Blood. 1964; 23: 216
        • Morse B.S.
        • Stohlman Jr., F.
        Regulation of erythropoiesis. XVIII. The effect of vincristine and erythropoietin on bone marrow.
        J. Clin. Invest. 1966; 45: 1241
      1. Reissmann, K. R., and Samorapoompichit, S.: Effect of erythropoietin on proliferation of erythroid stem cells in the absence of transplantable colony-forming units, Blood. In press.

        • Handler E.S.
        • Varsa E.E.
        • Gordon A.S.
        Mechanisms of leukocyte production and release. V. Studies on the leukocytosis-inducing factor in the plasma of rats treated with typhoid-paratyphoid vaccine.
        J. Lab. Clin. Med. 1966; 67: 398
        • Valeriote F.A.
        • Bruce W.R.
        Comparison of the sensitivity of hematopoietic colony-forming cells in different proliferative states to vinblastine.
        J. Nat. Cancer Inst. 1967; 38: 393
        • Gurney C.W.
        • Hofstra D.
        • Mangalik A.
        Physiological studies of primitive hemopoietic cells.
        Argonne Cancer Res. Hosp. Rep. March, 1965; : 21
        • Knospe W.H.
        • Gregory S.A.
        • Fried W.
        • Sassetti R.J.
        • Trobaugh Jr., F.E.
        Effect of cyclophosphamide (CTX) on the proliferation of hematopoietic stem cells (HSC).
        Clin. Res. 1970; 18: 407