Research Article| Volume 76, ISSUE 4, P659-667, October 1970

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Attenuation of urinary erythropoietin activity under various conditions

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      Because of unpredictable stability of urinary erythropoietin, urine has been kept in the frozen state from the time of collection until the time of assay. Since erythropoietin assay often requires large volumes of urine and is performed at specialized laboratories, the collection and transport of urine in the frozen state has been difficult and cumbersome in the past. The purpose of this study was to develop a more convenient procedure for the storage and preservation of urinary erythropoietin. The attenuation of urinary erythropoietin activity was systematically tested under varying conditions of pH, temperature, and duration of storage, with and without the addition of phenol as a bacteriostatic agent. Urine samples from patients with high erythropoietin excretion were compared with urine exogenously seeded with erythropoietin. At near neutral or alkaline pH, and in the presence of phenol, urinary erythropoietin activity remains stable at room temperature for 7 days, and at 4 °C. for up to 30 days. Acid pH and absence of phenol cause a more rapid reduction in erythropoietin activity.
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