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Because of unpredictable stability of urinary erythropoietin, urine has been kept in the frozen state from the time of collection until the time of assay. Since erythropoietin assay often requires large volumes of urine and is performed at specialized laboratories, the collection and transport of urine in the frozen state has been difficult and cumbersome in the past. The purpose of this study was to develop a more convenient procedure for the storage and preservation of urinary erythropoietin. The attenuation of urinary erythropoietin activity was systematically tested under varying conditions of pH, temperature, and duration of storage, with and without the addition of phenol as a bacteriostatic agent. Urine samples from patients with high erythropoietin excretion were compared with urine exogenously seeded with erythropoietin. At near neutral or alkaline pH, and in the presence of phenol, urinary erythropoietin activity remains stable at room temperature for 7 days, and at 4 °C. for up to 30 days. Acid pH and absence of phenol cause a more rapid reduction in erythropoietin activity.
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- The relation of erythropoietin to anemia and polycythemia.in: Jacobson L.O. Doyle M. Erythropoiesis. Grune & Stratton, Inc, New York1962: 340
- Erythropoietic activity of untreated and deproteinized normal human plasma.in: ed. 4. Proc. Soc. Exp. Biol. Med. 118. 1965: 823
- Studies on erythropoiesis. XIII. A comparison of methods of bioassay of erythropoietin in human plasma.J. Lab. Clin. Med. 1960; 55: 67
- Urinary excretion of erythropoietin in normal men and women.Blood. 1966; 28: 344
- Erythropoietin excretion in normal man.Blood. 1966; 28: 354
- Erythropoietin in concentrates of urine from healthy persons.Brit. Med. J. 1965; 5436: 697
- Erythropoietin in the urine of normal and erythropoietically abnormal human beings.Blood. 1966; 28: 535
- Textbook of biochemistry. The Macmillan Company, New York1966: 701 ed. 4.
- A comparison of erythropoietin bioassays.in: Proc. Soc. Exp. Biol. Med. 110. 1962: 48
- Purification of sheep erythropoietin.in: Fed. Proc. 18. 1959: 236
- Relation between severity of anemia and erythropoietin titer in human beings.Blood. 1961; 18: 187
- Plasma and urinary levels of erythropoietin in Cooley's anemia.Ann. N. Y. Acad. Sci. 1964; 119: 561
- Studies on erythropoietin.in: Recent progress in hormone research. vol. 16. Academic Press, Inc, New York1960: 219
- Serum and urinary levels of erythropoietin in iron deficiency anemias.in: Proc. Soc. Exp. Biol. Med. 126. 1967: 615
- Polycythemic response in normal adult rats to a nonprotein plasma extract from anemic rabbits.Blood. 1954; 9: 734
- Mechanism of the erythropoietic effect of cobalt.Science. 1957; 125: 1085
- The international reference preparation of erythropoietin.Bull. W. H. O. 1966; 35: 751
- Renal erythropoietin excretion as a function of acid base balance.Ann. N. Y. Acad. Sci. 1968; 149: 570
- Erythropoietin activity and protein-protein interactions in human urine.Biochim. Biophys. Acta. 1965; 104: 218
Accepted: July 31, 1970
Received: March 23, 1970
☆This investigation was supported by United States Public Health Service Grant AM/CA-11609 and General Research Support Grant FR-05576 from the National Institutes of Health, Bethesda, Md.
© 1970 Published by Elsevier Inc.