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Abstract
Erythrocytes preserved in acid-citrate-dextrose (ACD) were wasted and suspended in
TRIS-Ringer's medium at 37 °C. Cells incubated after 2 weeks of storage contained
more nicotinamide-adenine dinucleotide (NADH) and less oxidized nicotinamideadenine
dinucleotide (NAD+) than cells incubated after a few days of storage. When erythrocytes were suspended
in medium lacking glucose or containing 0.5 mM sodium iodoacetate (IAA), a decrease
in NADH was noted. A corresponding increase in NAD+ was observed in cells that had been stored for 2 weeks, but not in cells stored for
short periods. Following exposure to 5 mM sodium fluoride (F), NAD+ declined more markedly in erythrocytes stored for longer periods. These changes may
be associated with P1 elevation during storage because they could be duplicated by incubating briefly stored
cells in high inorganic phosphate medium. Lactate elevation during storage cannot
account for these changes since experiments with high-lactate medium did not produce
comparable results.
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Article info
Publication history
Accepted:
August 15,
1970
Received:
April 8,
1970
Footnotes
☆Supported by United States Public Health Service Grants No. GM 11430-05 and GM 738-09.
Identification
Copyright
© 1970 Published by Elsevier Inc.
