Research Article| Volume 76, ISSUE 4, P668-677, October 1970

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Influence of prior storage on pyridine nucleotide metabolism of human erythrocytes incubated in vitro

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      Erythrocytes preserved in acid-citrate-dextrose (ACD) were wasted and suspended in TRIS-Ringer's medium at 37 °C. Cells incubated after 2 weeks of storage contained more nicotinamide-adenine dinucleotide (NADH) and less oxidized nicotinamideadenine dinucleotide (NAD+) than cells incubated after a few days of storage. When erythrocytes were suspended in medium lacking glucose or containing 0.5 mM sodium iodoacetate (IAA), a decrease in NADH was noted. A corresponding increase in NAD+ was observed in cells that had been stored for 2 weeks, but not in cells stored for short periods. Following exposure to 5 mM sodium fluoride (F), NAD+ declined more markedly in erythrocytes stored for longer periods. These changes may be associated with P1 elevation during storage because they could be duplicated by incubating briefly stored cells in high inorganic phosphate medium. Lactate elevation during storage cannot account for these changes since experiments with high-lactate medium did not produce comparable results.
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