This paper is only available as a PDF. To read, Please Download here.
A radioisotope dilution technique for measurement of inorganic pyrophosphate (PPi) in synovial fluid is described. Its precision based on duplicate measurements is ±4 μg per 100 ml. PPi levels in 20 of 23 joint fluids from patients with arthritis other than pseudogout clustered about a mean ± S.D. of 22 ± 6.3; 3 control fluids showed elevated values. Of 18 joint fluids from patients with calcium pyrophosphate crystal deposition disease (pseudogout), 15 showed inorganic PPi levels that were greater than 2 S.D. from the control mean, 2 were borderline, and one fell in the normal range. The mean ± S.D. of the group was 100.4 ± 93. Inorganic orthophosphate, total and ionized calcium, alkaline phosphomonoesterase activity against both p-nitrophenylphosphate and β-glycerophosphate, and alkaline phosphomonoesterase isozymes were not significantly different in joint fluids from control and pseudogout patients. Preliminary studies on the solubility of calcium pyrophosphate dihydrate miscocrystals in various solvents such as distilled water, Tris buffer, dialyzed and anaerobically processed fresh joint fluid and plasma are presented. The levels of PPi attained were of the same order of magnitude as those found in joint fluids from pseudogout patients. Magnesium ions and protein increased solubility; calcium and orthophosphate ions decreased it. Whether or not the PPi levels found in pseudogout fluid represent saturation levels in vivo cannot be answered from the present data.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The significance of calcium phosphate crystals in the synovial fluid of arthritic patients: the “pseudogout syndrome.” I. Clinical aspects.Ann Intern Med. 1962; 56: 711-737
- Articular chondrocalcinosis.Ann Rheum Dis. 1963; 22: 142
- Studies on pathological calcifications in human cartilage.J Bone Joint Surg. 1966; 48A: 309-326
- The roentgenographic aspects of pseudogout (articular chondrocalcinosis); an analysis of 20 cases.Am J Roentgenol. 1963; 90: 1248-1257
- Basal urinary pyrophosphate excretion in pseudogout.Arthritis Rheum. 1969; 12: 228-231
- Inorganic pyrophosphate in the plasma, urine and synovial fluid of patients with pyrophosphate arthropathy, chondrocalcinosis or pseudogout.Lancet. 1970; 2: 899-902
- A comparative study of roentgenographic techniques for detection of calcium pyrophosphate dihydrate deposits (pseudogout) in human cartilage.Am J Roentgenol. 1967; 99: 688-694
- Compensated polarized light microscopy.JAMA. 1968; 203: 508-512
Thomas WC, and Lewis AM: Inorganic pyrophosphate in serum and urine. Submitted for publication.
- The colorimetric determination of phosphorus.J Biol Chem. 1925; 66: 375-400
- Microdetermination of phosphorus.Anal Chem. 1956; 28: 1756-1758
- New dye method for direct photometric determination of calcium.Am J Clin Pathol. 1957; 27: 223-230
- Studies with ion-exchange calcium electrodes: the distribution of ionized calcium between blood and cerebrospinal fluid in man and dog.J Clin Invest. 1968; 47 (Abst.): 70
- The determination of metals in blood serum by atomic absorption spectroscopy I.Spectrochem Acta. 1960; 16: 259-272
- Characterization of tissue and serum alkaline phosphatase.Clin Chim Acta. 1966; 14: 156-165
- Protein measurement with the Folin reagent.J Biol Chem. 1951; 193: 265
Sörensen LB: Personal communication.
- Hypophosphatasia associated with calcium pyrophosphate dihydrate deposits in cartilage.Arthritis Rheum. 1970; 13: 381-388
- Pyrophosphatase activity of mammalian alkaline phosphatase.Lancet. 1965; 2: 1018
- Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase.Biochem J. 1967; 105: 155
- Alkaline orthophosphatase and inorganic pyrophosphatase activities in human serum.Nature. 1967; 214: 842-843
- Pseudogout with low values of alkaline phosphatase in the synovial fluid.Ann Intern Med. 1970; 73: 751-756
- Synovial fluid pyrophosphate phosphohydrolase (PPPH) in pseudogout, gout, and rheumatoid arthritis.Arthritis Rheum. 1969; 12 (Abst.): 298
- Inhibition of human erythrocyte pyrophosphatase activity by calcium, cupric and ferrous ions.Arthritis Rheum. 1970; 13 (Abst.): 354
- Chondrocalcinosis articularis. II. Genetic study.Ann Rheum Dis. 1963; 22: 142-151
- Polyarticular and familial chondrocalcinosis.Arthritis Rheum. 1970; 13: 197-213
- Chondrocalcinosis (pseudogout syndrome). A family study.JAMA. 1964; 188: 867-871
- Calcium pyrophosphate crystal deposition disease (pseudogout, articular chondrocalcinosis).in: Hollander JL Arthritis and allied conditions. Lea & Febiger, Philadelphia1965: 113-118
- Les arthropathies des hemochromatoses.Rev Rhumatisme Malad Osteoartic. 1964; 31: 479-485
- The arthropathy of idiopathic haemochromatosis.Quart J Med. 1968; 37: 171-182
- Pseudogout syndrome (articular chondrocalcinosis).Bull Rheum Dis. 1964; 14: 331-334
Accepted: May 26, 1971
Received: January 29, 1971
☆Supported by United States Public Health Service Research Grant AM-13069 and Training Grant AM-05621.
© 1971 Published by Elsevier Inc.