This paper is only available as a PDF. To read, Please Download here.
Oxalate and glycolate were synthesized from 14C-glyoxylate by leukocytes, erythrocytes, and dialyzed hemolysate preparations. Oxalate decarboxylase was used in a convenient and reliable assay of oxalate produced. Leukocytes were approximately 10 to 20 times as active in oxalate and glycolate synthesis, respectively, as erythrocytes. Data submitted to indicate that the erythrocytic enzyme system is lactic dehydrogenase (LDH) include nicotinamide, adenine dinucleotide dependence, pH curve, simultaneous glycolate and oxalate synthesis, inhibition by oxalate and oxamate, and coincidence of isoenzyme activities for lactate and glyoxylate. Coincidence of glyoxylate and lactate oxidation by LDH isoenzymes was also shown in leukocyte preparations. These findings are discussed in terms of the implications of the coupling by LDH of glyoxylate oxidation to oxalate and its reduction to glycolate or the competitive reduction of another substrate. The implications for the development of a pharmacologic inhibitor of oxalate synthesis are also presented.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Studies on primary hyperoxaluria. II. Urinary oxalate, glycolate, and glyoxylate measurement by isotope dilution methods.J Lab Clin Med. 1965; 65: 677-687
- The variation of urinary oxalate excretion with age.J Lab Clin Med. 1969; 73: 901-908
- The biosynthesis and turnover of oxalate in normal and hyperoxaluric subjects.J Clin Invest. 1960; 39: 1337-1344
Williams HE, Johnson G, and Smith LH: Unpublished observations.
- The mechanism of the renal excretion of oxalate in the dog.Clin Sci. 1962; 22: 43-52
- The renal clearance of oxalic acid in normal subjects and in patients with primary hyperoxaluria.Invest Urol. 1963; 1: 87-93
- Role of ether-soluble organic acids in the cation-anion balance in plants.Plant Physiol. 1943; 18: 224-238
- Primary hyperoxaluria.Medicine. 1964; 43: 315-345
- Primary hyperoxaluria.in: Stanbury JB Wyngaarden JB Frederickson DS The metabolic basis of inherited disease. ed. 3. McGraw-Hill Book Co., Inc, New York1971
- Studies on primary hyperoxaluria. III. Transamination reactions of glyoxylate in human tissue preparations.J Lab Clin Med. 1967; 70: 494-502
- Leukocyte preparations from human blood: evaluation of their morphologic and metabolic state.J Lab Clin Med. 1962; 59: 779-791
- The determination of lactate dehydrogenase isoenzymes using the microzone cellulose acetate system.Clin Biochem. 1967; 1: 34-41
- Enzymatic oxalate determination in urine.Clin Chem. 1963; 9: 334-339
- The formation of oxalic acid from the side chain of aromatic amino acids in the rat.Biochim Biophys Acta. 1969; 184: 404-411
- Catalysis of both oxidation and reduction of glyoxylate by pig heart lactate dehydrogenase isozyme 1.J Biol Chem. 1970; 245: 1675-1681
- The metabolism of glyoxylate in blood from normal subjects and patients with primary hyperoxaluria.Clin Sci. 1968; 34: 97-110
- Lactic dehydrogenase. V. Inhibition by oxamate and by oxalate.J Biol Chem. 1959; 234: 1143-1148
- Primary hyperoxaluria: a defect in glyoxylate metabolism.J Clin Invest. 1962; 41 (Abst.): 1358
- Variations in the lactic dehydrogenase of vertebrate erythrocytes.J Gen Physiol. 1962; 45: 553-565
- Metabolic basis of phagocytic activity.Physiol Rev. 1962; 42: 143-168
- Studies of glycine oxidation in rat tissues.Arch Biochem Biophys. 1953; 42: 257-270
- The action of crystalline lactate dehydrogenase from rabbit muscle on glyoxylate.Biochim Biophys Acta. 1969; 177: 421-426
- Oxidation of glyoxylic acid to oxalic acid by glycolic acid oxidase.J Biol Chem. 1961; 236: 1280-1284
- L-glyceric aciduria: a new genetic variant of primary hyperoxaluria.N Engl J Med. 1968; 278: 233-239
- Hyperoxaluria in L-glyceric aciduria: a possible pathogenic mechanism.Science. 1971; 171: 390-391
- Porcine heart lactate dehydrogenase: optical rotatory dispersion, thermodynamics, and kinetics of binding reactions.J Biol Chem. 1969; 244: 4375-4381
- Rat liver lactate dehydrogenase. III. Kinetics and specificity.J Biol Chem. 1964; 239: 2991-2997
- The role of glycolysis in the growth of tumor cells. IV. The basis of glucose toxicity in oxamate-treated, cultured cells.J Biol Chem. 1965; 240: 2791-2796
- The effect of oxamate on glycolysis in intact ascites tumor cells. I. Kinetic evidence for a dual glycolytic system.Biochim Biophys Acta. 1970; 208: 189-202
- Stoffwechsel der weissen Blutzellen.Z Naturforsch. 1958; 13b: 515-516
- Inhibition of lactic dehydrogenase: a new approach to cancer chemotherapy.in: 1st edition. Surg Forum. 14. 1963: 128-130
Accepted: May 12, 1971
Received: February 10, 1971
☆This work was supported in part by Grant AM-09406 from the United States Public Health Service.
© 1971 Published by Elsevier Inc.