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The determination of serum vitamin B12 concentrations, unsaturated B12 binding capacity, and total B12 binding capacity of whole serum and of the separate transcobalamin I and transcobalalamin II fractions has shown that the increased B12 binding capacity of serum proteins in chronic myeloid leukemia is due mainly to an increase in transcobalamin I. Transcobalamin II is relatively unchanged. Serum B12 concentrations and unsaturated B12 binding capacity are only slightly elevated in acute myeloblastic leukemia; total binding capacity of transcobalamin I is within the normal range. The findings observed in acute promyelocytic leukemia are similar to those found in chronic myeloid leukemia. These observations suggest that the excessive amounts of transcobalamin I in chronic myeloid leukemia are produced by proliferating and differentiated myeloid cells beginning at the promyelocytic stage. Transcobalamin I is decreased markedly following the administration of cytotoxic agents.
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Accepted: April 28, 1971
Received: October 30, 1970
☆This work constitutes part of the Ph.D. program of B. Rachmilewitz.
© 1971 Published by Elsevier Inc.