Nonleukemic elevation of serum vitamin B12 and B12-binding capacity levels resembling that in chronic myelogenous leukemia

Ralph Carmel; Charles A. Coltman

doi:10.5555/uri:pii:0022214371902277

Research Article| Volume 78, ISSUE 2, P289-297, August 1971

Nonleukemic elevation of serum vitamin B₁₂ and B₁₂-binding capacity levels resembling that in chronic myelogenous leukemia

Ralph Carmel
Ralph Carmel
Correspondence
Reprint requests: Dr. Ralph Carmel, MRC Experimental Haematology Unit, St. Mary's Hospital Medical School, London W, 2, England.
Affiliations
From the Aerospace Medical Laboratory (Clinical) and the Hematology-Oncology Service, Wilford Hall USAF Medical Center Lackland AFB, Texas, USA
Search for articles by this author
Charles A. Coltman Jr.
Charles A. Coltman Jr.
Affiliations
From the Aerospace Medical Laboratory (Clinical) and the Hematology-Oncology Service, Wilford Hall USAF Medical Center Lackland AFB, Texas, USA
Search for articles by this author

DOI:https://doi.org/10.5555/uri:pii:0022214371902277

This paper is only available as a PDF. To read, Please Download here.

Abstract

In vitro serum vitamin B₁₂ abnormalities characteristic of chronic myelogenous leukemia were found in 5 patients without leukemia. All had leukocytosis, and 3 transiently had immature myeloid cells in peripheral blood. The B₁₂ abnormalities were: (1) marked elevation of alpha-globulin B₁₂-binding capacity in all subjects and (2) high serum B₁₂ levels in 3 patients with high-normal levels in 2. Beta-globulin binding was normal in 2 but elevated in the others. Electrophoretic and reticulocyte uptake studies also demonstrated the findings seen in chronic myelogenous leukemia. Of 3 patients studied at different stages, one's abnormalities disappeared as leukocytosis subsided, one's improved similarly but quickly rebounded as leukocytosis returned, and the third remained abnormal as leukocytosis persisted. A patient recovering from agranulocytosis due to chemotherapy was studied serially. The B₁₂ abnormalities paralleled the recovery phase to the exact day, returning to normal as leukocytosis subsided. High alpha-globulin B₁₂ binding and, often, serum B₁₂ levels resembling those in chronic myelogenous leukemia, thus, are not infrequently found in nonleukemic leukocytosis. The exact mechanisms responsible remain unknown. However, it is apparent that the abnormality is not an expression of the leukemic process itself and is not diagnostic for the disease.

To read this article in full you will need to make a payment

Purchase one-time access:

One-time access price info

For academic or personal research use, select 'Academic and Personal'
For corporate R&D use, select 'Corporate R&D Professionals'

Subscribe:

Already a print subscriber? Claim online access

Already an online subscriber? Sign in

Register: Create an account

Institutional Access: Sign in to ScienceDirect

References

- Beard MF
- Pitney WR
- Sanneman EH
Serum concentrations of vitamin B₁₂ in patients suffering from leukemia.
Blood. 1954; 9: 789-794
- Google Scholar
- Mollin DL
- Ross GIM
Serum vitamin B₁₂ concentrations in leukaemia and in some other haematological conditions.
Brit J Haemat. 1955; 1: 155-172
- Google Scholar
- Miller A
- Sullivan JF
The in vitro binding of cobalt-60-labeled vitamin B₁₂ by normal and leukemic sera.
J Clin Invest. 1958; 37: 556-566
- Google Scholar
- Miller A
- Sullivan JF
Electrophoretic studies of the vitamin B₁₂-binding protein of normal and chronic myelogenous leukemia serum.
J Clin Invest. 1959; 38: 2135-2143
- Google Scholar
- Mendelsohn RS
- Watkin DM
- Horbett AP
- et al.
Identification of the vitamin B₁₂-binding protein in the serum of normals and of patients with chronic myelocytic leukemia.
Blood. 1958; 13: 740-747
- Google Scholar
- Hall CA
- Finkler AE
Measurement of the amounts of the individual vitamin B₁₂-binding proteins in plasma. II. Abnormalities in leukemia and pernicious anemia.
Blood. 1966; 27: 618-622
- Google Scholar
- Gottlieb CW
- Retief FP
- Pratt PW
- et al.
Correlation of B₁₂-binding proteins with disorders of B₁₂ metabolism.
J Clin Invest. 1966; 45: 1016
- Google Scholar
- Meyer LM
- Cronkite EP
- Miller IF
- et al.
Co⁶⁰ vitamin B₁₂-binding capacity of human leukocytes.
Blood. 1962; 19: 229-235
- Google Scholar
- Simons K
- Weber T
The vitamin B₁₂-binding protein in human leukocytes.
Biochim Biophys Acta. 1966; 117: 201-208
- Google Scholar
- Stenman V-H
- Simons K
- Gräsbeck R
Vitamin B₁₂-binding protein in normal and leukemic human leukocytes and sera.
Scand J Lab Clin Invest. 1968; 21: 202-210
- Google Scholar
- Carmel R
- Coltman CA
Radioassay for serum vitamin B₁₂ with the use of saliva as the vitamin B₁₂ binder.
J Lab Clin Med. 1969; 74: 967-975
- Google Scholar
- Gottlieb C
- Lau KS
- Wasserman LR
- et al.
apid charcoal assay for intrinsic factor (IF), gastric juice-unsaturated B₁₂ binding capacity, antibody to IF, and serum-unsaturated B₁₂-binding capacity.
Blood. 1965; 25: 875-884
- Google Scholar
- Silverstein E
- Herbert V
Rapid determination of vitamin B₁₂-binding alpha and beta globulin in serum.
Blood. 1968; 31: 518-521
- Google Scholar
- Retief P
- Gottlieb CW
- Herbert V
Delivery of Co⁵⁷B₁₂ to erythrocytes from alpha and beta globulin of normal, B₁₂-deficient, and chronic myeloid leukemia serum.
Blood. 1967; 29: 837-851
- Google Scholar
- Simons K
Vitamin B₁₂ binders in human body fluids and blood cells.
Comment Biol Soc Sci Fenn. 1964; 27: 5
- Google Scholar
- Beard MF
- Pitney WR
- Sanneman EH
- et al.
Serum concentrations of vitamin B₁₂ in acute leukemia.
Ann Intern Med. 1954; 41: 323-327
- Google Scholar
- Erdmann-Oehlecker S
- Heinrich HC
Der Vitamin B₁₂-Stoffwechsel bei Hämoblastosen.
Clin Chim Acta. 1956; 1: 269-286
- Google Scholar
- Rachmilewitz M
- Izak G
- Hochman A
- et al.
Serum vitamin B₁₂ in leukemias and malignant lymphomas.
Blood. 1957; 12: 804-813
- Google Scholar
- Meyer LM
- Bertcher RW
- Cronkite EP
- et al.
Co⁶⁰ vitamin B₁₂-binding capacity of serum in persons with hematologic disorders, various medical diseases, and neoplasms.
Acta Med Scand. 1961; 169: 557-575
- Google Scholar
- Mikkonen L
- Finkler AE
- Hall CA
A diagnostic test for chronic myelogenous leukemia based on abnormalities of B₁₂-binding.
Acta Med Scand. 1967; 181: 591-596
- Google Scholar
- Hall CA
- Finkler AE
Vitamin B₁₂-binding protein in polycythemia vera plasma.
J Lab Clin Med. 1969; 73: 60-69
- Google Scholar
- Carmel R
- Coltman CA
Serum vitamin B₁₂ binding in nonleukemic leukocytosis.
Clin Res. 1970; 18: 40
- Google Scholar
- Carmel R
- Coltman CA
Serum vitamin B₁₂-binding capacity and muramidase changes with cyclic neutropenia induced by cytosine arabinoside.
Blood. 1971; 37: 31-39
- Google Scholar
- Olesen H
- Andersen MP
- Amris A
Serum vitamin B₁₂-binding capacity in patients with anemia.
Scand J Haemat. 1968; 5: 235-240
- Google Scholar
- Corcino J
- Krauss S
- Waxman S
- et al.
Release of vitamin B₁₂—binding protein by human leukocytes in vitro.
J Clin Invest. 1970; 49: 2250-2255
- Google Scholar
Carmel R: Vitamin B₁₂—binding protein abnormality in subjects without myeloproliferative disease. II. The presence of a third B₁₂—binding protein in serum. Submitted for publication.
- Google Scholar

Article info

Publication history

Accepted: March 31, 1971

Received: October 14, 1970

Footnotes

☆This study was supported by United States Air Force funds made available through AFR No.169-6.

Identification

DOI: https://doi.org/10.5555/uri:pii:0022214371902277

Copyright

ScienceDirect

Access this article on ScienceDirect

Property	Value
Status
Version
Ad File
Disable Ads Flag
Environment
Moat Init
Moat Ready
Contextual Ready
Contextual URL
Contextual Initial Segments
Contextual Used Segments
AdUnit
SubAdUnit
Custom Targeting
Ad Events
Invalid Ad Sizes