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Abstract
A modified assay of neutrophil function, which utilizes lysostaphin, is described.
Lysostaphin is a rapid-acting muralytic enzyme specific for S. aureus. Lysostaphin does not penetrate the neutrophils and can be rapidly inactivated. Exposure
of the neutrophil bacteria suspension to lysostaphin results in the elimination of
a high number of extracellular bacteria in minutes. This technique differentiates
defective phagocytosis from impaired intracellular killing more efficiently than other
known assay methods.
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References
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Article info
Publication history
Accepted:
May 12,
1971
Received:
January 13,
1971
Footnotes
☆This work was supported in part by Research Grant AI 03523 and Training Grant T1 AI 87 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service, and by a grant from the National Tuberculosis and Respiratory Disease Association and American Thoracic Society.
☆☆Presented in part at the Tenth Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill., Oct. 18, 1970.
Identification
Copyright
© 1971 Published by Elsevier Inc.
