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Research Article| Volume 81, ISSUE 1, P77-84, January 1973

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The determination of factor X in tissue homogenates. Evidence for the inactivation of Russell's viper venom by tissue homogenates in the presence of plasma

  • Ian L. Woolf
    Footnotes
    Affiliations
    From the Thorndike Memorial Laboratory, Harvard Medical Unit, Boston City Hospital, Boston, Mass. U.S.A.

    From the Department of Medicine, Harvard Medical School, Boston, Mass. U.S.A.
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  • Ruby S. Kipnes
    Affiliations
    From the Thorndike Memorial Laboratory, Harvard Medical Unit, Boston City Hospital, Boston, Mass. U.S.A.

    From the Department of Medicine, Harvard Medical School, Boston, Mass. U.S.A.
    Search for articles by this author
  • Bernard M. Babior
    Correspondence
    Reprint requests: Dr. Bernard M. Babior, New England Medical Center Hospital, 171 Harrison Ave., Boston, Mass. 02111.
    Footnotes
    Affiliations
    From the Thorndike Memorial Laboratory, Harvard Medical Unit, Boston City Hospital, Boston, Mass. U.S.A.

    From the Department of Medicine, Harvard Medical School, Boston, Mass. U.S.A.
    Search for articles by this author
  • Author Footnotes
    ∗ Research Fellow in Medicine, Harvard Medical School, Boston, Mass.
    ∗∗ Recipient of a Research Career Development Award from the National Institute of Arthritis and Metabolic Diseases.
DOI:https://doi.org/10.5555/uri:pii:0022214373902734
The determination of factor X in tissue homogenates. Evidence for the inactivation of Russell's viper venom by tissue homogenates in the presence of plasma
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      Abstract

      Determinations of the factor X levels of tissue homogenates by means of Russell's viper venom were found to yield artifactually low results. Investigation of this finding showed that the procoagulant activity of Russell's viper venom was destroyed by incubation of the venom with plasma plus the postmitochondrial supernatant from tissue homogenates. Homogenates from liver, spleen, and kidney were capable of destroying this activity. The role of this tissue-dependent process in the pathogenesis of envenomation by Russell's viper venom was discussed, and the significance of this activity in the determination of tissue factor X levels was commented on. Based on these results, a method was developed for the accurate determination of tissue factor X levels.
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      References

        • Macfarlane RG
        Russell's viper venom, 1934–1964.
        Br J Haematol. 1967; 7: 437-451
        • Colman RW
        The effect of proteolytic enzymes on bovine factor V. Kinetics of activation and inactivation by bovine thrombin.
        Biochemistry. 1969; 8: 1438-1445
        • Borchgrevink GF
        • Pool JG
        • Stormorken H
        A new assay for factor V (proaccelerin-accelerin) using Russell's viper venom.
        J Lab Clin Med. 1960; 55: 625-632
        • Prentice CR
        • Ratnoff OD
        The action of Russell's viper venom on factor V and the prothrombin-converting principle.
        Br J Haematol. 1969; 16: 291-302
        • Schiffman S
        • Theodor I
        • Rapaport SI
        Separation from Russell's viper venom of one fraction reacting with factor X and another reacting with factor V.
        Biochemistry. 1969; 8: 1397-1405
        • Bachmann F
        • Duckert F
        • Geiger M
        • et al.
        Differentiation of the factor VII complex. Studies on the Stuart-Prower factor.
        Thromb Diath Haemorrh. 1957; 1: 169-194
        • Babior BM
        • Kipnes RS
        Vitamin K-dependent formation of factor VII by a cell-free system from rat liver.
        Biochemistry. 1970; 9: 2564-2569
        • Hogeboom GH
        Fractionation of cell components of animal tissues.
        Meth Enzymol. 1955; 1: 16-19
        • Hjort P
        • Rapaport SI
        • Owren PA
        A simple, specific one-stage prothrombin assay using Russell's viper venom in cephalin suspension.
        J Lab Clin Med. 1955; 46: 89-97
        • McKay DG
        Disseminated Intravascular Coagulation.
        in: Harper and Row, New York1965: 360

      Article info

      Publication history

      Accepted: October 4, 1972
      Received: June 16, 1972

      Footnotes

      ☆This research was supported in part by Public Health Service Grants AM-09115, FR-0076, and AM-5413.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0022214373902734

      Copyright

      © 1973 Published by Elsevier Inc.

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