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Determinations of the factor X levels of tissue homogenates by means of Russell's viper venom were found to yield artifactually low results. Investigation of this finding showed that the procoagulant activity of Russell's viper venom was destroyed by incubation of the venom with plasma plus the postmitochondrial supernatant from tissue homogenates. Homogenates from liver, spleen, and kidney were capable of destroying this activity. The role of this tissue-dependent process in the pathogenesis of envenomation by Russell's viper venom was discussed, and the significance of this activity in the determination of tissue factor X levels was commented on. Based on these results, a method was developed for the accurate determination of tissue factor X levels.
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Accepted: October 4, 1972
Received: June 16, 1972
☆This research was supported in part by Public Health Service Grants AM-09115, FR-0076, and AM-5413.
© 1973 Published by Elsevier Inc.