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Abstract
In a previous study, evidence of material which appeared to have renal vasodilator
properties, and which inhibited renal tubular water reabsorption was found in the
plasma of dogs with shock. Some similarities to the action of vasodilator prostaglandins
(PG) are pointed out, and the present studies describe efforts to isolate prostaglandins
from shock plasma and normal plasma. Various extraction and chromatographic procedures
were used, and extracts were examined spectrophotometrically. Vasodepressor potency
of extracts were assayed in rats. Spectrophotometer peaks were found in shock plasma
but not normal plasma extracts at 278 and 217 nm. Extracts of shock plasma consistently
showed greater vasodepressor activity than extracts of normal plasma from the same
dog. These findings together with preliminary chromatographic studies suggested the
presence of a prostaglandin of the A series. More critical chromatographic examination
of extracts of large volumes of shock and normal plasma permitted the demonstration
of spots which migrated identically with pure PGE1 in both extracts, but only in shock
plasma extracts were spots migrating with pure PGA1 found.
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Article info
Publication history
Accepted:
September 29,
1972
Received:
May 26,
1972
Footnotes
☆Supported in part by a grant from the Eastern Ohio Chapter of the American Heart Association.
☆☆Part of these data were presented at the Alza Corporation Conference on Prostaglandins in Cellular Biology and the Inflammatory Process, Carmel, Calif., Oct. 24 to 26, 1971.
Identification
Copyright
© 1973 Published by Elsevier Inc.
