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Abstract
The present experiments measured the amount of activator peptide present in an inflammatory
granuloma during its evolution in vivo. Cotton pellets were implanted subcutaneously
in male Sprague-Dawley rats, and the resulting inflammatory granulomas were harvested
at intervals after implantation. The activator peptide concentration of cotton pellet
granulomas (units per 100 μg DNA) was elevated at day 4, reached a peak at day 7,
and returned to very low levels by 14 days. The hyaluronic acid (HA) concentration
of the granuloma was also greatest on day 7, while the concentration of sulfated MPS
tended to be highest by 14 days. The molecular weight (intrinsic viscosity) of hyaluronate
in the early granuloma was significantly greater than that found at 14 days and later.
Collagen was present in high concentration by 7 days. At 4 days polymorphonuclear
leukocytes were a major portion of the cell population, but by 7 days the dominant
cell types were fibrocytes and foreign-body giant cells. The elevated level of connective
tissue activating peptide (CTAP) found early in the inflammatory process is in keeping
with its postulated role as a regulator of the transition from the exudative phase
of inflammation to the reparative phase. The concomitant peak concentration of HA
in the granuloma is in agreement with the known in vitro actions of connective tissue
activating peptide.
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Article info
Publication history
Accepted:
September 29,
1972
Received:
June 23,
1972
With the technical assistance of EMILY L. DORSTEWITZ, JAMES C. RITCHIE and SUSAN F. SMITHFootnotes
☆This investigation was supported by United States Public Health Grant AM-10728 and by a grant from the Michigan Chapter of the Arthritis Foundation.
Identification
Copyright
© 1973 Published by Elsevier Inc.
