Research Article| Volume 81, ISSUE 1, P95-104, January 1973

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Connective tissue activation. V. The flux of connective tissue activating peptide during acute inflammation

  • C.William Castor
    Reprint requests: Dr. C. William Castor, Department of Internal Medicine, Rackham Arthritis Research Unit, University of Michigan Medical Center, Ann Arbor, Mich. 48104.
    From the Department of Internal Medicine and the Rackham Arthritis Research Unit, University of Michigan Medical Center, Ann Arbor, Mich. U.S.A.
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      The present experiments measured the amount of activator peptide present in an inflammatory granuloma during its evolution in vivo. Cotton pellets were implanted subcutaneously in male Sprague-Dawley rats, and the resulting inflammatory granulomas were harvested at intervals after implantation. The activator peptide concentration of cotton pellet granulomas (units per 100 μg DNA) was elevated at day 4, reached a peak at day 7, and returned to very low levels by 14 days. The hyaluronic acid (HA) concentration of the granuloma was also greatest on day 7, while the concentration of sulfated MPS tended to be highest by 14 days. The molecular weight (intrinsic viscosity) of hyaluronate in the early granuloma was significantly greater than that found at 14 days and later. Collagen was present in high concentration by 7 days. At 4 days polymorphonuclear leukocytes were a major portion of the cell population, but by 7 days the dominant cell types were fibrocytes and foreign-body giant cells. The elevated level of connective tissue activating peptide (CTAP) found early in the inflammatory process is in keeping with its postulated role as a regulator of the transition from the exudative phase of inflammation to the reparative phase. The concomitant peak concentration of HA in the granuloma is in agreement with the known in vitro actions of connective tissue activating peptide.
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      1. Castor CW: Unpublished observations.