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Abstract
The serum content of antibodies to DNA and to RNA has been measured by means of a
solid-phase immunoassay using polynucleotides adsorbed to plastic cups. The technique
shows high specificity for measurement of antibody to double-stranded DNA and RNA,
and distinguishes antibody to single-stranded DNA from antibody to double-stranded
DNA. The technique avoids problems of anticomplementarity of sera, shows good resolving
capability, and allows recovery of the radioactively-labeled polynucleotide reagent.
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Article info
Publication history
Accepted:
September 29,
1972
Received:
April 14,
1972
Footnotes
☆This study was supported by Grant AMO 1229, National Institutes of Health, Bethesda, Md., a grant (No. 14) from the Research Committee of the School of Medicine, University of California, San Francisco, Calif., and by a grant from The Seeing Eye, Inc., Morristown, N. J.
Identification
Copyright
© 1973 Published by Elsevier Inc.