Research Article| Volume 81, ISSUE 1, P122-132, January 1973

A solid-phase immunoassay for antibody to DNA and RNA

  • Margaret Tan
    Reprint requests: Mrs. Margaret Tan, University of California, San Francisco Medical Center, Rheumatic Disease Group, HSE 671, San Francisco, Calif. 94122.
    From the Rheumatic Disease Group, Department of Medicine, University of California-San Francisco, San Francisco, Calif. U.S.A.
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  • Wallace V. Epstein
    From the Rheumatic Disease Group, Department of Medicine, University of California-San Francisco, San Francisco, Calif. U.S.A.
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      The serum content of antibodies to DNA and to RNA has been measured by means of a solid-phase immunoassay using polynucleotides adsorbed to plastic cups. The technique shows high specificity for measurement of antibody to double-stranded DNA and RNA, and distinguishes antibody to single-stranded DNA from antibody to double-stranded DNA. The technique avoids problems of anticomplementarity of sera, shows good resolving capability, and allows recovery of the radioactively-labeled polynucleotide reagent.
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