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A relatively simple immunoassay for quantitative determination of human erythrocyte catalase is described. In hemolysates from 45 healthy individuals the enzyme concentration was 1.95 ± 0.37 μg per milligram Hb (range 1.37 to 2.82). The values obtained with the immunoassay were almost identical to those determined by the complicated enzymatic measurement (1.74 ± 0.34). The advantages of the immunoassay for a large number of determinations in various physiologic, genetic, and pathologic conditions are discussed.
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Accepted: September 29, 1972
Received: June 21, 1972
☆This investigation was supported by a grant from the Chief Scientist's Office, Ministry of Health, Israel.
© 1973 Published by Elsevier Inc.