Research Article| Volume 81, ISSUE 1, P133-139, January 1973

Specific immunoassay for quantitative determination of human erythrocyte catalase

  • Yoav Ben-Yoseph
    From the Pediatric Research Unit, Department of Pediatrics and Child Care, Hadassah University Hospital Medical School, Hebrew University, Jerusalem, Israel
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  • Emmanuel Shapira
    Reprint requests: Dr. E. Shapira, Pediatric Department, Hadassah University Hospital, P. O. B. 499, Jerusalem, Israel.
    From the Pediatric Research Unit, Department of Pediatrics and Child Care, Hadassah University Hospital Medical School, Hebrew University, Jerusalem, Israel
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  • Author Footnotes
    ∗ Work performed in partial requirements for Ph.D. degree at the Hebrew University. Jerusalem.
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      A relatively simple immunoassay for quantitative determination of human erythrocyte catalase is described. In hemolysates from 45 healthy individuals the enzyme concentration was 1.95 ± 0.37 μg per milligram Hb (range 1.37 to 2.82). The values obtained with the immunoassay were almost identical to those determined by the complicated enzymatic measurement (1.74 ± 0.34). The advantages of the immunoassay for a large number of determinations in various physiologic, genetic, and pathologic conditions are discussed.
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