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Abstract
Determination of isotope uptake by leukocytes in diluted whole blood incubated with
3H-thymidine proved to be a very sensitive method for detecting DNA-synthesizing cells
in the circulation. Among normal subjects, uptake per unit volume of blood did not
correlate with either lymphocyte or granulocyte counts, and probably reflected a low
level of “spontaneous blastogenesis” among circulating lymphocytes. Thymidine uptake
was normal or modestly elevated in a number of nonleukemic disease states. In chronic
lymphocytic leukemia, uptake values ranged from subnormal to grossly elevated levels,
were relatively stable, and correlated with aggressiveness of the disease. Low or
normal values uniformly predicted a favorable near-term course. In chronic myelocytic
leukemia, thymidine uptake appeared to be principally into immature granulocytes,
correlated with white blood cell count and percentage of immature cells, was acutely
affected by treatment, and could fluctuate rapidly and widely. Among patients in stable
“good” control of the disease, uptake values correlated with course during the subsequent
year. Measurement of in vitro leukocyte thymidine uptake can identify the presence
of proliferating cells in the circulation, not detectable by routine hematologic studies.
It should prove particularly useful in monitoring the status of patients with leukemia
and related disorders.
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References
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Article info
Publication history
Accepted:
July 9,
1974
Received:
March 18,
1974
Footnotes
☆Supported in part by United States Public Health Service Grant CA-12243.
Identification
Copyright
© 1974 Published by Elsevier Inc.
