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Abstract
Activities of 5 Krebs-Henseleit (KH) enzymes [carbamyl phosphate synthetase (CPS),
ornithine transcarbamylase (OT), argininosuccinic acid synthetase (ASS), argininosuccine
acid lyase (AS), and arginase (A)] were determined in liver tissue obtained at laparotomy
from 17 patients with alcoholic cirrhosis, and from 19 individuals without liver disease.
Specific activities of the 5 enzymes were calculated on the basis of 4 units of reference:
wet weight, total N, noncollagen N, and DNA. Specific activities of all 5 enzymes
were depressed in the cirrhotic liver. Degree of reduction was similar regardless
of which unit of reference was employed to calculate specific activity. Average extent
of depression for activity per milligram of DNA was 54 per cent, 37 per cent, 50 per
cent, 64 per cent, and 69 per cent for CPS, OT, ASS, AS, and A, respectively. Rate-limiting
enzymes for operation of KH cycle were CPS, ASS, and AS in both normal and cirrhotic
livers. Extent of loss of these three enzymes in cirrhotic liver measured in vitro
correlated with reduction in maximal rate of urea synthesis and with severity of NH4Cl intolerance measured in vivo.
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References
- Maximal rates of urea synthesis in normal and cirrhotic subjects.J Clin Invest. 1973; 52: 2241
- Studies on carbamyl phosphate-L-ornithine carbamyltransferase from ox liver.Biochem J. 1963; 87: 409
- Biosynthesis of urea. XI. Preparation and properties of crystalline argininosuccinase.J Biol Chem. 1965; 240: 3079
- Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver.J Biol Chem. 1959; 234: 1769
- Herditary metabolic disorders of the urea cycle.Adv Clin Chem. 1971; 14: 65
- On the determination of ornithine-carbamyltransferase activity.Clin Chim Acta. 1964; 9: 95
- A spectrophotometric method for the determination of urea.Clin Chim Acta. 1963; 8: 295
- New analytical procedure for the estimation of DNA with p-nitrophenylhydrazine.Anal Biochem. 1972; 47: 562
- Modification of a specific assay for hydroxyproline in urine.Anal Biochem. 1967; 19: 249
- Influence of pancreatic hormones on enzymes concerned with urea synthesis in rat liver.Biochem J. 1965; 94: 410
- Effect of adrenalectomy and of growth hormone on enzymes concerned with urea synthesis in rat liver.Biochem J. 1963; 87: 96
- Effect of azo dye carcinogenesis on enzymes concerned with urea synthesis in the rat.Biochem J. 1964; 91: 464
- Relation of blood glucose to blood ammonia and urea cycle enzymes.JAMA. 1967; 199: 135
- Quantitative determination of DNA in normal and abnormal liver.J. Pharmacol Sci. 1969; 58: 1036
- Zinc and collagen content of cirrhotic liver.Dig Dis. 1970; 15: 797
- Hemodynamic contrasts between selective and total portal-systemic decompression.Ann Surg. 1971; 173: 827
- Liver enzymes in the Eck fistula rat.Enzyme. 1972; 14: 353
Article info
Publication history
Accepted:
July 1,
1974
Received:
March 22,
1974
Footnotes
☆Supported in part by United States Public Health Service Grants AM 15736 and RR00039.
Identification
Copyright
© 1974 Published by Elsevier Inc.
