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Abstract
In recent reports, reovirus-like particles have been identified in stools of children
with diarrhea by using several different methods of preparing the stools for electron
microscopy. These papers have reported varying incidences of the reovirus-like agent
(RLA) in groups of patients with diarrhea. Since no standardized stool processing
method has been used, it is difficult to compare the incidence figures which have
been published. This study evaluates two commonly used methods to determine which
would be more sensitive for use in future clinical studies. Stool samples from 72
Mexican children who had acute-onset diarrhea were processed for reading on the electron
microscope by the pseudoreplica technique and by differential centrifugation. Reovirus-like
particles were seen in 25 samples (34.7 percent) when the results of both methods
were combined. With the pseudoreplica technique 22 samples (30.6 percent) contained
the viral particles; with differential centrifugation 21 (29.2 percent) had particles.
This study demonstrates that the two methods are equally reliable for demonstrating
reovirus-like particles in stool samples. We believe that the pseudoreplica technique,
which is quicker and less expensive than differential centrifugation, is the preferred
method for clinical studies of diarrhea associated with reovirus-like particles.
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References
- Detection of a new virus by electron microscopy of fecal extracts from children with acute gastroenteritis.Lancet. 1974; 1: 149-151
- Importance of a new virus in acute sporadic enteritis in children.Lancet. 1975; 1: 242-246
- The viral flora of the faeces as seen by electron microscopy.J Clin Pathol. 1974; 27: 603-608
- Acute gastroenteritis associated with reovirus-like particles.J Clin Pathol. 1974; 27: 608-614
- Reoviruslike agent in stools: Association with infantile diarrhea and development of serologic tests.Science. 1974; 185: 1049-1053
- Orbivirus acute gastroenteritis of infancy.Lancet. 1974; 1: 1241-1244
- “Rotavirus” in gastroenteritis.Lancet. 1974; 2: 726
- Viruses in acute summer gastroenteritis in black infants.Lancet. 1975; 1: 1093-1094
- Virus particles and gastro-enteritis in black and white children in Rhodesia.S Afr Med J. 1975; 49: 859-863
- Occurrence of “viral particles” in diarrhea: Houston, Texas, and Guatemala.N Engl J Med. 1975; 292: 644-645
- Occurrence of “viral particles” in diarrhea: Atlanta, Georgia.N Engl J Med. 1975; 292: 1297
- Preparation of viruses for electron microscopy.in: ed. 2. Methods in Cancer Research. Vol. 1. Academic Press, Inc, New York1967: 562-567
Article info
Publication history
Accepted:
June 1,
1976
Received:
January 6,
1976
Identification
Copyright
© 1977 Published by Elsevier Inc.
