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Abstract
Female LAF1 mice were given single or repeated injections of V. cholerae N'ase and the effects on circulating RBC surface charge and life span were determined.
Intravenous injection of N'ase caused a rapid decrease in RBC surface charge of approximately
14 percent, and survival of such treated cells was reduced by approximately one fifth
by virtue of an acceleration of senescence. When RBC's were treated in vitro with
N'ase, a comparable (14 to 17 percent) reduction in surface charge was seen. Such
cells, when injected into intact mice, showed a similar acceleration of senescence.
When N'ase was injected intravenously into splenectomized mice, RBC survival was similar
to that of controls. Intravenous injection of N'ase 1 hour before injection of labeled
RBC's did not alter RBC survival nor did it accelerate the clearance of carbon particles
by the RES. These results indicate that N'ase accelerates senescence in treated mouse
erythrocytes by acting on the RBC's and not by activating the RES. Absence of this
effect in splenectomized mice implicates the spleen as the sensor of the induced alterations
in surface charge. These results and those recently reported for treated RBC's in
the dog, rat, rabbit, and man suggest that at least a portion of the phenomenon of
RBC senescence may be related to the loss of RBC surface charge.
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Article info
Publication history
Accepted:
June 14,
1976
Received:
November 6,
1975
Footnotes
☆This work was supported, in part, by a Public Health Service Research Career Development Award (5-K4-HL-17693-03) from the National Heart and Lung Institute (Dr. Landaw), a Public Health Service Research Grant from the National Cancer Institute (CA-15184-01, Dr. Tenforde), by the United States Energy Research and Development Administration (ERDA) under Contract W-7405-eng-48, and by Research Funds from the V.A. Hospital, Syracuse, N. Y. (MRIS No. 5134-01).
☆☆Presented, in part, at the Western Section Meeting of the American Federation for Clinical Research, Carmel, Calif., Feb. 2, 1973.1
Identification
Copyright
© 1977 Published by Elsevier Inc.
