Original article| Volume 99, ISSUE 1, P46-55, January 1982

Effects of nitrogen, phosphorus, and caffeine on calcium balance in women

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      The effects of different levels of nitrogen, phosphorus, and caffeine intake on calcium balance and on certain of its components were assessed in 170 studies in normal middle-aged, but still premenopausal women. Statistically significant negative associations with calcium balance were found for nitrogen and for caffeine, but no effect could be found for phosphorus. Higher nitrogen intakes were associated with proportionately higher levels of urinary calcium; higher phosphorus intake was associated with slightly lower levels of urinary calcium but also with slightly more intestinal secretion of calcium. Since these two effects were opposite in direction, there was no net association of different phosphorus intakes with calcium balance. Caffeine intake was associated with higher levels of both urinary calcium and intestinal calcium secretion. None of the three intake variables was associated with differences in calcium absorption efficiency. Both the nitrogen and the caffeine effects were proportional to intake. The magnitude of the effects observed was such that a 50% increase in intake of nitrogen above the group mean intake value would be predicted to result in calcium balance shift of −0.032 gm/day. For caffeine, the corresponding calcium balance shift would be predicted to be −0.006 gm/day.


      total intestinal calcium secretion (TIC) ()
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        • Heaney RP
        • Recker RR
        • Saville PD
        Calcium balance and calcium requirements in middleaged women.
        Am J Clin Nutr. 1977; 30: 1603
        • Recker RR
        • Saville PD
        • Heaney RP
        The effect of estrogens and calcium carbonate on bone loss in postmenopausal women.
        Ann Intern Med. 1977; 87: 649
        • Horsman A
        • Gallagher JC
        • Simpson M
        • Nordin BEC
        Prospective trial of oestrogen and calcium in postmenopausal women.
        Br Med J. 1977; 2: 789
        • Johnson NE
        • Alcantara EN
        • Linkswiler HM
        Effect of protein intake on urinary and fecal calcium and calcium retention of young adult males.
        J Nutr. 1970; 100: 1425
        • Walker RM
        • Linkswiler HM
        Calcium retention in the adult human male as affected by protein intake.
        J Nutr. 1972; 102: 1297
        • Anand CR
        • Linkswiler HM
        Effect of protein intake on calcium balance of young men given 500 mg calcium daily.
        J Nutr. 1974; 104: 695
        • Schwartz R
        • Woodcock NA
        • Blakely JD
        • MacKellar I
        Metabolic responses of adolescent boys to two levels of dietary magnesium and protein. II. Effect of magnesium and protein level on calcium balance.
        Am J Clin Nutr. 1973; 26: 519
        • Margen S
        • Chu J-Y
        • Kaufman NA
        • Callaway DH
        Studies in calcium metabolism. I. The calciuretic effect of dietary protein.
        Am J Clin Nutr. 1974; 27: 584
        • Chu J-Y
        • Margen S
        • Costa FM
        Studies in calcium metabolism. II. Effects of low calcium and variable protein intake on human calcium metabolism.
        Am J Clin Nutr. 1975; 28: 1028
        • Jowsey J
        Osteoporosis, its nature and the role of diet.
        Postgrad Med. August, 1976; 60: 75
        • Jowsey J
        Metabolic Diseases of Bone.
        in: ed. 6. Saunders Monographs in Clinical Orthopaedics. vol. I. W. B. Saunders Co, Philadelphia1977 (chap. 29)
        • Jowsey J
        Osteoporosis: dealing with a crippling bone disease of the elderly.
        Geriatrics. July, 1977; 32: 41
        • Daniell HW
        Osteoporosis and the slender smoker.
        Arch Intern Med. 1976; 136: 298
        • Heaney RP
        • Recker RR
        • Saville PD
        Menopausal changes in calcium balance performance.
        J Lab Clin Med. 1978; 92: 953
        • Fiske CH
        • SubbaRow P
        The colorimetric determination of phosphorus.
        J Biol Chem. 1925; 66: 375
        • deGrazia JA
        • Ivanovich P
        • Fellows H
        • Rich C
        A double isotope method for measurement of intestinal absorption of calcium.
        J Lab Clin Med. 1965; 66: 829
        • Heaney RP
        • Saville PD
        • Recker RR
        Calcium absorption as a function of calcium intake.
        J Lab Clin Med. 1975; 85: 881
        • Heaney RP
        • Skillman TG
        Secretion and excretion of calcium by the human gastrointestinal tract.
        J Lab Clin Med. 1964; 64: 29
        • Snedecor GW
        • Cochran WG
        Statistical Methods.
        ed. 6. Iowa State University Press, Ames, Iowa1967
        • Ostle B
        Statistics in Research.
        Iowa State University Press, Ames, Iowa1954 (chap. 8)
        • Abraham S
        • Carroll MS
        • Dresser CM
        • Johnson CL
        Vital and Health Statistics.
        DHEW Publication 77ndash;1647. 1977; (Series 11, No. 202)
        • Spencer H
        • Kramer L
        • Osis D
        Effect of a high protein (meat) intake on calcium metabolism in man.
        Am J Clin Nutr. 1978; 31: 2167
        • Spencer H
        • Kramer L
        • Osis D
        • Norris C
        Effect of phosphorus on the absorption of calcium and on the calcium balance in man.
        J Nutr. 1978; 108: 447
        • Jowsey J
        • Reiss E
        • Canterbury JM
        Long-term effects of high phosphate intake on parathyroid hormone levels and bone metabolism.
        Acta Orthop Scand. 1974; 45: 801
        • Goldsmith RS
        • Richards R
        • Dube WJ
        • et al.
        Metabolic effects and mechanism of action of phosphate supplements.
        in: Hioco DJ Phosphate et Métabolisme Phosphocalcique (Proceedings of International Symposium). Laboratories Sandoz, Paris1970
        • Lemann J
        • Adams ND
        • Gray RW
        Urinary calcium excretion in human beings.
        N Engl J Med. 1979; 301: 535
        • Kim Y
        • Linkswiler H
        Effect of level of protein intake on calcium metabolism and on parathyroid and renal function in the adult human male.
        J Nutr. 1979; 109: 1399
        • Schuette SA
        • Zemel MB
        • Linkswiler HM
        Studies on the mechanism of protein-induced hypercalciuria in older men and women.
        J Nutr. 1980; 110: 305