Original article| Volume 102, ISSUE 5, P694-698, November 1983

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Membrane-bound hemoglobin in the erythrocytes of sickle cell anemia

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      The thoroughly washed ghosts of erythrocytes from normal subjects and patients with sickle cell anemia were assayed for their content of total protein, total globin, hemoglobin, and α and β chains by chemical and electrophoretic techniques. Total protein, globin, and hemoglobin were significantly greater in sickle cell than in normal ghosts. Ghosts prepared from different density fractions of sickle red cells showed no significant differences in protein, globin, or hemoglobin, though membrane-bound globin was highest in the most dense cells. In both whole populations and density fractions of sickle red cells, the majority of the membrane-bound globin retained its heme. Alpha and βS chains were present in equal amounts in the sickle cell membranes.


      (RBCs) (red blood cells), (SS) (sickle cell anemia), (ISCs) (irreversibly sickled cells)
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