This paper is only available as a PDF. To read, Please Download here.
Abstract
The thoroughly washed ghosts of erythrocytes from normal subjects and patients with
sickle cell anemia were assayed for their content of total protein, total globin,
hemoglobin, and α and β chains by chemical and electrophoretic techniques. Total protein,
globin, and hemoglobin were significantly greater in sickle cell than in normal ghosts.
Ghosts prepared from different density fractions of sickle red cells showed no significant
differences in protein, globin, or hemoglobin, though membrane-bound globin was highest
in the most dense cells. In both whole populations and density fractions of sickle
red cells, the majority of the membrane-bound globin retained its heme. Alpha and
βS chains were present in equal amounts in the sickle cell membranes.
Abbreviations:
(RBCs) (red blood cells), (SS) (sickle cell anemia), (ISCs) (irreversibly sickled cells)To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Translational ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Irreversibly sickled cells and red cell survival in sickle cell anemia. A study with both DF32P and 51Cr.Am J Med. 1978; 64: 253
- Erythrocyte adherence to endothelium in sickle-cell anemia.N Engl J Med. 1980; 302: 992
- Abnormalities in membrane phospholipid organization in sickled erythrocytes.J Clin Invest. 1981; 67: 1643
- Denatured hemoglobin in sickle erythrocytes.J Clin Invest. 1977; 59: 633
- Membrane alterations in irreversibly sickled cells: hemoglobin-membrane interaction.J Supramolec Struct. 1978; 9: 537
- Membrane-bound hemichrome in density-separated cohorts of normal (AA) and sickled (SS) cells.J Lab Clin Med. 1982; 99: 25
- Cation permeability alterations during sickling: relationship to cation composition and cellular dehydration of irreversibly sickled cells.Blood. 1978; 51: 983
- Progressive inhibition of the Ca pump and Ca:Ca exchange in sickle red cells.Nature. 1980; 284: 561
- Influence of temperature and method of centrifugation on separation of erythrocytes.J Lab Clin Med. 1973; 82: 334
- Protein measurement with the Folin phenol reagent.J Biol Chem. 1951; 193: 265
- A modification of the benzidine method for measurement of hemoglobin in plasma and urine.Blood. 1956; 11: 380
- Measurement of hemoglobin chains bound to the erythrocyte membrane.J Lab Clin Med. 1980; 96: 318
- Classification and localization of hemoglobin binding sites on the red blood cell membrane.Biochemistry. 1977; 16: 5593
- The binding of hemoglobins to membranes of normal and sickle erythrocytes.Biochim Biophys Acta. 1975; 375: 422
- Mechanical stability of hemoglobin subunits: an abnormality in βS subunits of sickle hemoglobin.Biochem Biophys Res Commun. 1974; 57: 780
- An acid protease in human erythrocytes and its localization in the inner membrane.Eur J Biochem. 1979; 96: 221
Article info
Publication history
Accepted:
June 21,
1983
Received:
May 4,
1983
Footnotes
☆This research was supported by grant AM 28210 from the National Institutes of Health and by a grant from the American Heart Association, Texas Affiliate.
Identification
Copyright
© 1983 Published by Elsevier Inc.
