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The thoroughly washed ghosts of erythrocytes from normal subjects and patients with sickle cell anemia were assayed for their content of total protein, total globin, hemoglobin, and α and β chains by chemical and electrophoretic techniques. Total protein, globin, and hemoglobin were significantly greater in sickle cell than in normal ghosts. Ghosts prepared from different density fractions of sickle red cells showed no significant differences in protein, globin, or hemoglobin, though membrane-bound globin was highest in the most dense cells. In both whole populations and density fractions of sickle red cells, the majority of the membrane-bound globin retained its heme. Alpha and βS chains were present in equal amounts in the sickle cell membranes.
Abbreviations:(RBCs) (red blood cells), (SS) (sickle cell anemia), (ISCs) (irreversibly sickled cells)
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Accepted: June 21, 1983
Received: May 4, 1983
☆This research was supported by grant AM 28210 from the National Institutes of Health and by a grant from the American Heart Association, Texas Affiliate.
© 1983 Published by Elsevier Inc.