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Abstract
Methods of synovial fluid collection and processing known to affect cryoprotein formation
were examined to investigate the proposed role of fibronectin in synovial fluid cryoprecipitation.
Fibronectin, a nonimmunoglobulin, noncomplement synovial fluid protein was present
in all resolubilized synovial fluid cryoproteins studied. Radiolabeled fibronectin
was precipitated from rheumatoid synovial fluid to a significantly greater extent
(10%) than from noninflammatory (osteoarthritic) synovial fluid (2.8%), normal plasma
(1.3%), or normal serum (0.5%) (p < 0.01). Clotting of synovial fluid reduced fibronectin
concentration 44% and resulted in a reduction in the amount and percent incorporation
of fibronectin into cryoprotein, whereas heparinization and hyaluronidase treatment
increased cryoprecipitable fibronectin. Affinity depletion of synovial fluid fibronectin
resulted in loss of C1q and reduction in IgG in the cryoprotein; however, fibronectin,
C1q, and IgG could not be co-eluted from affinity matrices of gelatin and protein
A-Sepharose. Cryoprotein formation from pathologic synovial fluid depends in part
on fibronectin and appears to involve interactions between fibronectin and fibrinogen
as well as immunoglobulin complexes and complement components.
Abbreviations:
(Fn) (fibronectin), (SDS-PAGE) (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), (Kd) (kilodalton), (PBS) (phosphate-buffered saune), (RA) (rheumatoid arthritis), (OA) (osteoarthritis), (EDTA) (ethylenediamine tetraacetic acid), (DTT) (dithiothreitol)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
June 27,
1983
Received:
March 10,
1983
Footnotes
☆Supported in part by U.S.P.H.S. General Clinical Research Center Grant RR 00318 and grants from the Kroc Foundation and the Arthritis Foundation, New York Chapter.
☆☆Presented in part at the Association of American Physicians, American Society for Clinical Investigation, and American Federation for Clinical Research Meeting, Washington, D. C. May 1982 and appeared in abstract form (Clin Res 30:469A 1982).
Identification
Copyright
© 1983 Published by Elsevier Inc.
