Advertisement
Original article| Volume 102, ISSUE 5, P773-778, November 1983

Download started.

Ok

Nephelometric measurement of human serum amyloid P component (SAP)

  • Morie A. Gertz
    Affiliations
    From the Thorndike Memorial Laboratory and Division of Medicine, Boston City Hospital, Boston, Mass., USA

    From the Arthritis Center, Boston University School of Medicine, Boston, Mass., USA

    From the Mayo Clinic and Mayo Medical School, Rochester, Minn., USA
    Search for articles by this author
  • Martha Skinner
    Correspondence
    Reprint requests: Dr. Martha Skinner, Arthritis Section, E-337, Boston University School of Medicine, 75 East Newton St., Boston, Mass. 02118.
    Affiliations
    From the Thorndike Memorial Laboratory and Division of Medicine, Boston City Hospital, Boston, Mass., USA

    From the Arthritis Center, Boston University School of Medicine, Boston, Mass., USA

    From the Mayo Clinic and Mayo Medical School, Rochester, Minn., USA
    Search for articles by this author
  • Alan S. Cohen
    Affiliations
    From the Thorndike Memorial Laboratory and Division of Medicine, Boston City Hospital, Boston, Mass., USA

    From the Arthritis Center, Boston University School of Medicine, Boston, Mass., USA

    From the Mayo Clinic and Mayo Medical School, Rochester, Minn., USA
    Search for articles by this author
  • Robert A. Kyle
    Affiliations
    From the Thorndike Memorial Laboratory and Division of Medicine, Boston City Hospital, Boston, Mass., USA

    From the Arthritis Center, Boston University School of Medicine, Boston, Mass., USA

    From the Mayo Clinic and Mayo Medical School, Rochester, Minn., USA
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Serum amyloid P component (SAP) is a normal constituent of human serum. A rate nephelometric technique has been developed that can measure this protein more rapidly and reproducibly than previously described methods. Materials required are simple and commercially available. Studies on 30 patients with amyloidosis show no difference in SAP levels from control patients. Rate nephelometry is a simple technique that could expand the ability to measure SAP beyond the research setting.

      Abbreviations:

      (SAP) (serum amyloid P component), (AP) (P-component), (RIA) (radioimmunoassay), (AL) (primary amyloidosis), (AA) (secondary amyloidosis), (AF) (familial amyloidosis), (CRP) (C-reactive protein)
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Skinner M
        • Cohen AS
        • Shirahama T
        • Cathcart ES
        P-component (pentagonal unit) of amyloid: isolation, characterization, and sequence analysis.
        J Lab Clin Med. 1974; 84: 604
        • Benson MD
        • Skinner M
        Amino terminal sequence of amyloid P-component isolated from serum.
        J Lab Clin Med. 1978; 92: 78
        • Westermark P
        • Shirahama T
        • Skinner M
        • Brun A
        • Cameron R
        • Cohen AS
        Immunohistochemical evidence for the lack of amyloid P-component in some intracerebral amyloids.
        Lab Invest. 1982; 46: 457
        • Pepys MB
        • Dash AL
        • Munn EA
        • Feinstein A
        • Cohen AS
        • Gewurz H
        • Osmand AP
        • Painter RH
        Isolation of amyloid P-component (protein AP) from normal serum as a calcium dependent binding protein.
        Lancet. 1977; 1: 1029
        • Osmand AP
        • Freidenson B
        • Gewurz H
        • Painter RH
        • Hofman T
        • Shelton E
        Characterization of C-reactive protein and the complement subcomponent Clt as homologous protein displaying cyclic pentametric symetry (pentraxins).
        in: Proc Natl Acad Sci USA. 74. 1977: 739
        • Skinner M
        • Vaitukaitis JL
        • Cohen AS
        • Benson MD
        Serum amyloid P-component levels in amyloidosis, connective tissue diseases, infection, and malignancy as compared to normal serum.
        J Lab Clin Med. 1979; 94: 633
        • Laurell CB
        Electroimmunoassay.
        Scand J Clin Lab Invest. 1972; 29: 21
        • Pepys MB
        • Dash AC
        • Markham RE
        • Thomas HC
        • Williams BD
        • Petrie A
        Comparative clinical study of protein SAP (amyloid P-component) and C-reactive protein in serum.
        Clin Exp Immunol. 1978; 32: 199
        • Strachan AF
        • Johnson PM
        Protein SAP (serum amyloid P-component) in Waldenstroms macroglobulinemia, multiple myeloma and rheumatic diseases.
        J Clin Lab Immunol. 1982; 8: 153
        • Jensson O
        • Bjornsson G
        • Arnason A
        • Birgisdottry
        • Pepys MB
        Serum amyloid P-component and C-reactive protein in serum of healthy Icelanders and members of an Icelandic family with macroglobulinemia.
        Acta Med Scand. 1982; 211: 341
        • Sternberg JC
        A rate nephelometer for measuring specific proteins by immunoprecipitin reactions.
        Clin Chem. 1977; 23: 1456
        • Hoffken K
        • Schmidt CG
        Quantification of immune complexes by nephelometry.
        Methods Enzymol. 1981; 74: 628
        • Skinner M
        • Pepys MB
        • Cohen AS
        • Heller LM
        • Lian JB
        Studies on amyloid protein AP.
        in: Glenner GG Costa PP deFreitas F Amyloid and Amyloidosis. Excerpta Medica Foundation, Amsterdam1980: 384
        • Lowry OH
        • Rosebrough NJ
        • Farr AL
        • Randall RJ
        Protein measurement with the Folin phenol reagent.
        J Biol Chem. 1951; 193: 265
        • Ruiz-Arenas R
        • Lepe-Zuniga JL
        • Ruiz-Arquelles A
        • deRuiz NL
        • Marin-Loped A
        • Ruiz-Reyes G
        Laser nephelometry quantitation of fibrinogen related antigens.
        Am J Clin Pathol. 1981; 76: 284
        • Pott G
        • Meyering M
        • Voss B
        • Karges HE
        • Sieber A
        Rapid determination of fibronectin by laser nephelometry.
        J Clin Chem Clin Biochem. 1980; 18: 893
        • Cohen AS
        • Wegelius O
        Classification of amyloid: 1979–1980.
        Arthritis Rheum. 1980; 23: 644
        • Skinner M
        • Sipe JD
        • Yood RA
        • Shirahama T
        • Cohen AS
        Characterization of P-component (AP) isolated from amyloidotic tissue. Half-life studies of human and murine AP.
        Ann NY Acad Sci. 1982; 389: 190
        • Pepys MB
        • Dash AC
        • Fletcher TC
        • Richardson N
        • Munn EA
        • Feinstein A
        Analogues in other mammals and in fish of human plasma proteins, C-reactive protein and amyloid P-component.
        Nature. 1978; 273: 168