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Abstract
The relationship between platelet abnormalities and vessel wall changes in diabetes
is not known. We have examined the time course of alterations in in vitro platelet
function and endothelial damage, as assessed by measurement of plasma levels of von
Willebrand factor (VIIIR:WF) and factor VIII-related antigen (VIIIR:Ag), in streptozotocin-induced
diabetic rats. Platelet aggregation and the platelet release reaction in response
to ADP, thrombin, and collagen were measured in suspensions of washed platelets prepared
from rats 3, 7, 14, or 28 days after induction of diabetes and in control animals.
Platelets from diabetic animals showed enhanced aggregation response to ADP as early
as 3 days after induction of diabetes and became hyperresponsive to thrombin after
7 days, compared to control platelets. Thrombin-induced release of serotonin was greater
in platelets from diabetic animals at 14 days. Collagen-induced responses were not
different at any time studied. VIIIR:WF was determined by ristocetin-induced platelet
agglutination time in gel-filtered platelets, and VIIIR:Ag was determined by immunoelectrophoretic
technique. VIIIR:WF and VIIIR:Ag were significantly enhanced in plasma from rats at
28 days after induction of diabetes and VIIIR:Ag was enhanced in plasma from rats
at 14 days after induction of diabetes, but at the earlier times studied, neither
were different from values in plasma from control-treated rats. Changes in VIIIR:WF
and VIIIR:Ag therefore occurred later than the changes in platelet function. Plasma
cholesterol concentrations were not significantly different at any of the times studied,
but plasma triglyceride concentrations were significantly increased at 3 days and
remained increased with further durations of diabetes. This may have contributed to
the observed platelet and vessel wall changes. If these in vitro alterations reflect
in vivo behavior, then platelet alterations occur before vessel wall changes and therefore
do not appear to be a consequence of such changes in experimental diabetes mellitus.
Abbreviations:
(VIIIR:WF) (von Willebrand factor), (VIIIR:Ag) (factor VIII-related antigen), (ACD) (acid-citrate-dextrose)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
July 12,
1983
Received:
June 17,
1983
Footnotes
☆Supported by grants from the South Carolina State Appropriation for Biomedical Research and the Veterans Administration.
Identification
Copyright
© 1983 Published by Elsevier Inc.
