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Abstract
Ambient hydrogen ion concentration modulates the effects of angiotensin II (AII) on
adrenal aldosterone secretion, but the mechanism of this modulation is unknown. We
examined the influence of pH on AII receptors and responses in bovine adrenal glomerulosa
cells. Lowering pH from 7.4 to 6.8 increased AII binding 20.5% and increased maximal
AII-stimulated aldosterone secretion 43%. By contrast, at pH 8.0, AII binding and
stimulation of aldosteronogenesis fell by 56.6% and 39%, respectively. Effects on
AII binding intermediate to these changes were observed at pH 7.1 and 7.7. Similar
effects of altered pH were observed on AII binding to a crude membrane fraction of
bovine glomerulosa cells. Analysis indicated that pH primarily affected receptor number
rather than affinity. In studies of proposed postreceptor mediators of AII actions,
pH had no effect on AII stimulation of phosphatidylinositol turnover or AII inhibition
of calcium influx. The results show that pH affects AII interaction with its receptors.
The larger magnitude of the change in aldosterone compared with receptor binding suggests
that a postreceptor step is also altered by pH; however, this postreceptor step is
not reflected in calcium influx or phospholipid turnover.
Abbreviations:
AII (angiotensin II), cyclic AMP (adenosine 3′,5′-cyclic monophosphate), EDTA (ethylenediaminetetraacetic acid), HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), PI (phosphatidylinositol)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
March 26,
1986
Received:
August 6,
1985
Footnotes
☆Supported by the Veterans Administration and by a grant-in-aid from the American Diabetes Association-Wisconsin Affiliate.
Identification
Copyright
© 1986 Published by Elsevier Inc.
