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Ambient hydrogen ion concentration modulates the effects of angiotensin II (AII) on adrenal aldosterone secretion, but the mechanism of this modulation is unknown. We examined the influence of pH on AII receptors and responses in bovine adrenal glomerulosa cells. Lowering pH from 7.4 to 6.8 increased AII binding 20.5% and increased maximal AII-stimulated aldosterone secretion 43%. By contrast, at pH 8.0, AII binding and stimulation of aldosteronogenesis fell by 56.6% and 39%, respectively. Effects on AII binding intermediate to these changes were observed at pH 7.1 and 7.7. Similar effects of altered pH were observed on AII binding to a crude membrane fraction of bovine glomerulosa cells. Analysis indicated that pH primarily affected receptor number rather than affinity. In studies of proposed postreceptor mediators of AII actions, pH had no effect on AII stimulation of phosphatidylinositol turnover or AII inhibition of calcium influx. The results show that pH affects AII interaction with its receptors. The larger magnitude of the change in aldosterone compared with receptor binding suggests that a postreceptor step is also altered by pH; however, this postreceptor step is not reflected in calcium influx or phospholipid turnover.
Abbreviations:AII (angiotensin II), cyclic AMP (adenosine 3′,5′-cyclic monophosphate), EDTA (ethylenediaminetetraacetic acid), HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), PI (phosphatidylinositol)
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- Aldosterone production by isolated glomerulosa cells: modulation of sensitivity to angiotensin II and ACTH by extracellular potassium concentration.Endocrinology. 1977; 100: 481
- Multiplicative interaction between angiotensin II and K concentration in stimulation of aldosterone.Am J Physiol. 1984; 247: E328-E335
- Further studies on the relationship between potassium and sodium levels and adrenocortical activity.Endocrinology. 1978; 102: 1061
- Studies on cyclic nucleotides in the adrenal gland. VIII. Effects of angiotensin on adenosine 3′,5′-monophosphate and steroidogenesis in the adrenal cortex.Endocrinology. 1978; 103: 1361
- The role of calcium in the stimulation of aldosterone production by adrenocorticotropins, angiotensin II, and potassium in isolated glomerulosa cells.Endocrinology. 1979; 105: 327
- Angiotensin-induced steroidogenesis in rabbit adrenal: effects of pH and calcium.Mol Cell Endocrinol. 1979; 13: 159
- Role of Ca2+ in response of adrenal glomerulosa cells to angiotensin II, ACTH, K+, and ouabain.Am J Physiol. 1981; 241: E42
- Influence of cations on kinetics of angiotensin II binding to adrenal, renal, and smooth muscle receptors.Hypertension. 1982; 4: III79
- Correlation of the biological activity and solution conformation of [Asp, Ile5]-and [Phe4, Tyr8]-angiotensin II.in: Proc Natl Acad Sci USA. 75. 1978: 3630
- Calcium and angiotensin tachyphylaxis in rat uterine smooth muscle.Am J Physiol. 1975; 228: 1423
- Acid-base effects on aldosterone secretion (Abstr).Physiologist. 1984; 27: 206
- Angiotensin alters 45Ca++ fluxes in bovine adrenal glomerulosa cells.in: Proc Natl Acad Sci USA. 78. 1981: 3044
- Aspects of angiotensin action in the adrenal. Key roles for calcium and phosphatidyl inositol.Hypertension. 1982; 4: 52
- Effects of angiotensin-II and potassium on phospholipid metabolism in the adrenal zona glomerulosa.J Biol Chem. 1981; 256: 11093
- Correlation between cytosolic free Ca2+ and aldosterone production in bovine adrenal glomerulosa cells.J Biol Chem. 1984; 259: 8863
- Rapid effects of angiotensin II on polyphosphoinositide metabolism in the rat adrenal glomerulosa.Endocrinology. 1984; 114: 302
- Specific inhibition of receptors for angiotensin II and angiotensin III in adrenal glomerulosa.Biochem Pharmacol. 1980; 29: 927
- Jaffe BM Behrman HR Methods of hormone radioimmunoassay. Academic Press, New York1979: 889
- The attraction of proteins for small molecules and ions.NY Acad Sci. 1949; 51: 660
- Statistical analysis of radioimmunoassays and immunoradiometric (labelled antibody) assay: a generalized, weighted, iterative, least-squares method for logistic curve-fitting.in: Hunter Jr, OB Radioassays: clinical concepts. GD Searle & Co, Skokie, Ill1974: 209
- Cholesteryl hemisuccinate alters membrane fluidity, angiotensin receptors, and responses in bovine adrenal glomerulosa cells.Life Sci. 1983; 32: 1573
- Adrenal sensitivity to angiotensin II and undiscovered aldosterone stimulating factors in hypertension.J Steroid Biochem. 1979; 11: 1043
- Properties of angiotensin II receptors in the bovine and rat adrenal cortex.J Biol Chem. 1974; 249: 825
- The effect of acidosis and alkalosis on in vitro aldosterone production.Metabolism. 1983; 32: 697
- pH modulation of ligand binding to adrenal mitochondrial cytochrome P-450scc.J Biol Chem. 1982; 257: 4731
- Study on the influence of tyrosine deprotonation on the myotropic action of angiotensin II.Can J Physiol Pharmacol. 1979; 57: 317
- s-Cis and s-transisomerism of the his-pro peptide bond in angiotensin and thyroliberin analogues.Biochemistry. 1979; 18: 1952
- Conformations of angiotensin II and enkephalin related to NMR.Biochem Biophys Res Commun. 1979; 91: 534
- Angiotensin and bradykinin interactions with phospholipids.Biochim Biophys Acta. 1979; 586: 357
- The quantitation of carbamino adduct formation of angiotensin II and bradykinin.Biophys J. 1978; 24: 765
- Effect of pH on binding kinetics and biological effect of insulin in rat adipocytes.J Biol Chem. 1981; 256: 6250
Accepted: March 26, 1986
Received: August 6, 1985
☆Supported by the Veterans Administration and by a grant-in-aid from the American Diabetes Association-Wisconsin Affiliate.
© 1986 Published by Elsevier Inc.