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Abstract
The effect of mast cells and mast cell granules on macrophge O2̄ release as determined by cytochrome c reduction was studied. In vitro activation
of mast cells before macrophage activation caused a decrease in O2̄-mediated cytochrome c reduction. This decrease was proportional to mast cell activation
and reached 80% to 100% when mast cell mediator release was 40% to 50%. Incubation
of isolated mast cell granules with macrophages before activation also inhibited O2̄-mediated cytochrome c reduction in a dose-dependent manner. Mast cell granule-mediated
inhibition of cytochrome c reduction was not caused by histamine, serotonin, or any
other dialyzable components but was found to be caused by the scavenging of O2̄ by mast cell granule—bound superoxide dismutase. Macrophage uptake of sulfur 35-labeled
mast cell granules, electron microscopic localization of mast cell granules in the
macrophage phagosomes, and the abrogation of mast cell granule effect when the cells
were preincubated at 0 ° C indicate that the effect was associated with the adherence
or phagocytosis (or both) of mast cell granules. These results suggest that mast cell
granules interact with macrophages and that granule Superoxide dismutase scavenges
O2̄ generated by the phagocytes.
Abbreviations:
4880 (condensation compound of N-methyl-p-methoxyphenethylamine and formaldehyde), DNP-BSA (dinitrophenyl bovine serum albumin), OZ (opsonized zymosan), EDTA (ethylenediaminetetraacetic acid), IgE (immunoglobulin E), TGD (Tyrode's buffer containing 0.1% gelatin and 30 μg/ml deoxyribonuclease)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
December 28,
1988
Received in revised form:
December 27,
1988
Received:
September 22,
1988
Footnotes
☆Supported by funds from the Joseph G. and Elizabeth E. Carey Arthritis Research Funds and from the Hinman Fund, and by Grant BRSG507-RR0573 from the National Institutes of Health.
Identification
Copyright
© 1989 Published by Elsevier Inc.
