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Abstract
The purpose of this study was to relate changes in collagen composition with the mechanical
properties of aortas of spontaneously hypertensive rats (SHRs). SHRs and normal Wistar-Kyoto
(WKY) rats were killed at ages 6 and 20 weeks, and their aortas were removed. A portion
of each aorta was used for determination of passive mechanical properties, and the
remainder was incubated with carbon 14-labeled proline containing medium for 18 hours
at 37 ° C. After incubation, the tissues were lyophilized, and allquots were used
for sequential extraction with neutral salt, acetic acid, and pepsin at 4 ° C. The
pepsin extracts that contained most of the collagen were used for characterization
of the collagen types and synthesis of collagen by the production of [14C]-labeled hydroxyproline. Collagen concentration decreased approximately 16% in both
6- and 20-week-old aortas, but collagen synthesis was about twofold higher in the
aortas from both 6- and 20-week-old SHRs. Although type V collagen represented a minor
fraction of total collagen (5%) in the aortas of WKY rats, it was twofold greater
in the hypertensive animals. This increment in type V collagen in SHR aortas was accompanied
by a reduction in the proportion of type I collagen, with no change in the proportion
of type III. Study of the passive mechanical properties of the tissues showed that
SHR aortas were stiffer compared with aortas of WKY rats, with a larger difference
at 20 weeks than at 6 weeks. These differences in passive stiffness did not correlate
with collagen concentration or types at the two ages. Overall, this study shows alterations
in collagen concentration and relative proportion of collagen types in SHR aortas
when compared with those from WKYs.
Abbreviations:
PSS (physiologic salt solution), SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), SHR (spontaneously hypertensive rat), WKY (Wistar-Kyoto rat)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
January 3,
1989
Received in revised form:
December 29,
1988
Received:
October 3,
1988
Footnotes
☆Supported by National Institutes of Health Grant HL 28476.
Identification
Copyright
© 1989 Published by Elsevier Inc.
