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Abstract
We performed studies involving large Incremental additions of calcium to bile to examine
the degree of CaCO3 supersaturation that can be achieved in bile, as compared with that in simple NaCl-NaHCO3 solutions. The addition of CaCl2 to bile increased total and free ionized calcium by from four to 12 times their original
values and by from two to three times the highest concentrations encountered in bile
in vivo. The increase in [Ca++] resulted in fourfold to 12-fold increases in the saturation indexes of bile, with
the saturation index reaching as high as 73.4 in a common duct sample. Despite the
markedly elevated CaCO3 saturation indexes, evidence of CaCO3 precipitation was not observed in either common duct or gallbladder bile over a 24-hour
period. For comparison, calcium added to NaCl-NaHCO3 solutions resulted in the precipitation of CaCO3 within 4 hours if saturation indexes were greater than 12. These results indicate
that native bile contains potent antinucleating factors that markedly inhibit CaCO3 precipitation, and they confirm our previous in vivo observations in canine common
duct bile.
Abbreviations:
K'sp (apparent solubility product of a salt), IP (ion product of a salt), Sl (saturation index)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
January 19,
1989
Received in revised form:
January 11,
1989
Received:
November 1,
1988
Footnotes
☆Supported by National Institutes of Health Grant DK 32130 from the National Institute of Diabetes, Digestive, and Kidney Diseases and the Veterans Administration Medical Research Service.
☆☆Presented in part at the annual meeting of the American Association for the Study of Liver Diseases, Chicago, Ill., November 1987. A preliminary report has been published (Hepatology 1987;7:1110).
Identification
Copyright
© 1989 Published by Elsevier Inc.
