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Abstract
We have studied the erythrocyte membrane phospholipid organization in hereditary high
red cell membrane phosphatidylcholine hemolytic anemia (HPCHA) and the response of
these red cells during incubation with sonicated suspensions of dimyristoylphosphatidylcholine
(DMPC). Although both the absolute and relative amounts of phosphatidylcholine were
elevated in these red cells, the relative distribution of phosphatidylcholine on both
sides of the membrane bilayer and the transbilayer mobility of phosphatidylcholine
were normal. HPCHA erythrocytes showed elevated absolute amounts per cell of both
protein and lipid and an increased cellular ratio of protein to phospholipid. Incubation
of normal red cells with DMPC led to the formation of echinocytes, followed by the
release of acetylcholinesterase-containing vesicles. Both echinocyte formation and
vesiculation were markedly reduced in red cells from patients with HPCHA. Studies
with red cells from patients with liver disease, cells that also have elevated relative
amounts of membrane phosphatidylcholine and increased amounts of lipid per cell, revealed
normal echinocyte formation and normal DMPC-induced vesiculation. We conclude that
the altered lipid composition of HPCHA erythrocytes per se is not responsible for
the observed reduction in DMPC-induced vesiculation, but that it is more likely the
result of a modification in the protein moiety of these cells. This putative protein
abnormality could enhance binding of phosphatidylcholine to red cell membranes and
could explain the elevated phosphatidylcholine content of HPCHA erythrocytes and their
inability to veslculate.
Abbreviations:
DMPC = dimyristoylphosphatidylcholine (), HPCHA = hereditary high phosphatidylcholine hemolytic anemia (), SDS = sodium dodecyl sulfate ()To read this article in full you will need to make a payment
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References
- Red cell vesiculation—a common membrane physiologic event.J Lab Clin Med. 1986; 108: 315-324
- Microvesicles from sickle erythrocytes and their relation to irreversible sickling.Br J Haematol. 1981; 47: 383-390
- Release of spectrin-free vesicles from human erythrocytes during ATP depletion.J Cell Biol. 1977; 73: 548-560
- Release of diacylgycerol-enriched vesicles from erythrocytes with increased intracellular [Ca2+].Nature. 1976; 261: 58-60
- Vesicle production on heated and stressed erythrocytes.Biochim Biophys Acta. 1978; 512: 318-330
- Effect of dimyristoylphosphatidylcholine on intact erythrocytes: release of spectrin-free vesicles without ATP depletion.Biochim Biophys Acta. 1981; 641: 79-87
- Release of vesicles containing acetylcholinesterase from erythrocyte membranes by treatment with dilauroylphosphatidylcholine.J Biochem. 1983; 93: 1691-1699
- Quantitative composition and characterization of the proteins in membrane vesicles released from erythrocytes by dimyristoylphosphatidylcholine. A membrane system without cytoskeleton.J Cell Biochem. 1982; 19: 179-191
- The influence of cellular ATP levels on dimyristoylphosphatidylcholine-induced release of vesicles from human erythrocytes.Biochim Biophys Acta. 1985; 821: 91-96
- Effect of cholesterol on dimyristoylphosphatidylcholineinduced vesiculation of human red blood cells.Biochim Biophys Acta. 1986; 855: 293-301
- Effect of sickling on dimyristoylphosphatidylcholine-induced vesiculation in sickle red blood cells.Biochim Biophys Acta. 1986; 855: 286-292
- Modulation of erythrocyte vesiculation by amphiphilic drugs.Biochim Biophys Acta. 1987; 901: 291-295
- Hereditary nonspherocytic hemolytic disease associated with an altered phospholipid composition of the erythrocytes.J Clin Invest. 1968; 47: 1375-1388
- Hereditary hemolytic anemia associated with abnormal membrane lipids: mechanism of accumulation of phosphatidylcholine.Blood. 1971; 38: 445-456
- Study of erythrocytes in a hereditary hemolytic syndrome: comparison with erythrocytes in lecithin:cholesterol acyltransferase deficiency.Scand J Haematol. 1980; 24: 122-130
- Spin label studies of erythrocytes with abnormal lipid composition: comparison of red cells in a hereditary hemolytic syndrome and lecithin:cholesterol acyltransferase deficiency.J Supramol Struct Cell Biochem. 1981; 15: 213-218
- Lipid analysis and fluidity studies by electron spin resonance of red cell membranes in hereditary high red cell membrane phosphatidylcholine hemolytic anemia.Blood. 1984; 64: 1129-1134
- Heinz body formation in red cell membrane disorders: its acceleration in membrane lipid abnormalities.Scand J Haematol. 1985; 35: 257-263
- Separation of erythrocytes according to age on a simplified density gradient.J Lab Clin Med. 1974; 84: 147-151
- Improved procedure for the extraction of lipids from human erythrocytes.J Lipid Res. 1965; 6: 428-431
- Quantitative two-dimensional thin-layer chromatography of blood phospholipids.Clin Chim Acta. 1969; 23: 457-463
- Two-dimensional thinlayer Chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.Lipids. 1970; 5: 494-496
- A new method for direct determination of serum cholesterol.J Lab Clin Med. 1953; 41: 486-492
- A new and rapid colometric determination of acetylcholinesterase activity.Biochem Pharmacol. 1961; 7: 88-95
- Organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases.Biochim Biophys Acta. 1975; 406: 83-96
- Shape changes in human erythrocytes induced by replacement of the native phosphatidylcholine with species containing various fatty acids.J Cell Biol. 1984; 99: 2260-2267
- Phosphatidylcholine exchange protein from beef liver.Methods Enzymol. 1983; 98: 581-586
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227. 1970: 680-685
- Investigation by crossed immunoelectrophoresis of membrane-cytoskeleton interactions in human erythrocyte membranes.in: Azzi A Brodbeck U Zahler P Enzymes, receptors and carriers of biological membranes. Springer-Verlag, Berlin1984: 44-51
- The immunochemical approach to the characterization of membrane proteins: human erythrocyte membrane proteins analyzed as a model system.Biochim Biophys Acta. 1976; 455: 66-89
- An analysis of lipoproteins, bile acids, and red cell membranes associated with target cells and spur cells in patients with liver disease.J Clin Invest. 1972; 51: 3182-3192
- Characteristics of the membrane defect in the hereditary stomatocytosis syndrome.Blood. 1975; 46: 337-356
- Erythrocyte lipid composition and sodium transport in human liver disease.Biochim Biophys Acta. 1978; 510: 168-176
- Decreased erythrocyte membrane fluidity and altered lipid composition in human liver disease.J Lipid Res. 1982; 23: 124-132
- Transbilayer distribution and mobility of phosphatidylcholine in intact erythrocyte membranes.Eur J Biochem. 1980; 103: 283-288
- Flip-flop rates of individual molecular species of phosphatidylcholine in the human red cell membrane.Biochim Biophys Acta. 1986; 855: 421-424
- Accelerated transbilayer movement of phosphatidylcholine in sickled erythrocytes. A reversible process.J Biol Chem. 1983; 258: 8435-8442
- Interactions between membrane skeleton proteins and the intrinsic domain of the erythrocyte membrane.Biochim Biophys Acta. 1982; 694: 331-352
- Interaction of a peripheral protein of the erythrocyte membrane, band 4.1, with phosphatidylserinecontaining liposomes and erythrocyte inside-out vesicles.Eur J Biochem. 1983; 130: 19-25
- Human erythrocyte protein 4.1 is a phosphatidylserine binding protein.J Clin Invest. 1987; 81: 255-260
- Regulation of the association of membrane skeletal protein 4.1 with glycophorin by a polyphosphoinositide.Nature. 1985; 318: 295-298
- Separation of the lipid bilayer from the membrane skeleton during echinocytic transformation of red cell ghosts.J Cell Biol. 1987; 12: 291a
Article info
Publication history
Accepted:
November 15,
1988
Received in revised form:
November 8,
1988
Received:
July 1,
1988
Footnotes
☆Supported in part by National Institutes of Health Grants HL27059, HL36255, and AM32094, by Grant 3,358-0.82 from the Swiss National Science Foundation (SNSF), and by a stipend from the SNSF to P.B.
Identification
Copyright
© 1989 Published by Elsevier Inc.
