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Abstract
A myeloma cell line, MM.1, has been established from the peripheral blood cells of
a patient with immunoglobulin A myeloma. MM.1 grows in suspension either singly or
in small clusters and secretes λ-light chain. Phenotyplcally, MM.1 cells lack most
B cell antigens, but they do express human leukocyte antigen DR, PCA-1, and T9 and
T10 antigens. Molecular analysis of MM.1 demonstrates that it is negative for the
presence of the Epsteln-Barr virus genome. Southern analysis of MM.1 detected a rearrangement
of the λ-light chain gene, and Northern analysis revealed high levels of λ gene expression.
Cytogenetic analysis of the MM.1 cell line revealed the presence of seven related
chromosomally abnormal cell lines characterized by numerical and structural aberrations,
and it revealed five nonclonal abnormal cells. The most notable abnormality is a reciprocal
translocation involving band q24.3 of chromosome 12 and band q32.3 of chromosome 14;
translocations involving 14q32 are frequently observed in neoplasms of B cell origin.
Abbreviations:
AANAE (acid naphthyl acetate esterase), ACP (acid phosphatase), ANAE (naphthyl acetate esterase), ANBE (naphthyl butyrate esterase), EBV (Epstein-Barr virus), ELISA (enzyme-linked immunoassay), HLA (human leukocyte antigen), mRNA (messenger ribonucleic acid), PAS (periodic acid Schiff), PBL (peripheral blood mononuclear cells)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
November 8,
1988
Received in revised form:
October 14,
1988
Received:
April 26,
1988
Footnotes
☆Supported in part by Department of Energy Contract DE-FG02-86ER60408 and Public Heatlh Service Grant CA 41644 (M.M.L.).
☆☆M.M.L. is a scholar of the Leukemia Society of America.
Identification
Copyright
© 1989 Published by Elsevier Inc.
