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Abstract
We compared the capacity of eight different intravenous immunoglobulin (IVIG) preparations
to opsonize streptococcal group A particles and to induce lumlnolenhanced chemiluminescence
in neutrophil granulocytes. Antibodies to the streptococcal group A carbohydrate (A-CHO)
antigen exposed on the particles were present in all preparations at high concentrations.
In some preparations, anti-A-CHO consisted predominantly of either immunoglobulin
G1 (IgG1) or IgG2, whereas in others, both IgG subclasses were equally represented.
Dilutions of the IVIGs and of a normal IgG reference preparation were adjusted for
their anti-A-CHO content, incubated with streptococcal particles, and exposed to neutrophil
granulocytes isolated from normal blood. The various IVIGs differed in their capacity
to elicit chemlluminesence signals. Some preparations, particularly those consisting
of chemically modified IgG molecules, showed little activity, whereas others were
comparable to the reference preparation. We concluded that to some extent such differences
could be related to the IgG subclass composition of the antibody. However, of greater
importance were the manufacturing procedures by which both the antigen-binding fragment
and crystalllzable fragment portions, and thus the functional integrity of the IgG
molecules, were more or less severely affected.
Abbreviations:
A-CHO = streptococcal group A carbohydrate (), EDTA = ethylenediaminetetraacetic acid (), ELISA = enzyme-linked immunosorbent assay (), Fab = antigen-binding fragment (), Fc = crystallizable fragment (), HEPES = N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (), IgG = immunoglobulin G (), MG = intravenous immunoglobulin (), PBS = phosphate-buffered saline solution (), PMA = phorbol 12-myristate 13-acetate ()To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
November 22,
1988
Received in revised form:
November 22,
1988
Received:
August 5,
1988
Footnotes
☆Supported by grants from the Central Laboratory of the Swiss Red Cross Blood Transfusion Service and from the Swiss National Science Foundation.
Identification
Copyright
© 1989 Published by Elsevier Inc.
