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Abstract
The charge-related determinants of albumin permeability have been the subject of much
controversy. We have examined the permeability of either isolated glomerular basement
membrane (GBM) or intact glomeruli in an in vitro filtration system to determine the
effects of biochemical modifications selected to alter the molecules commonly implicated
in restricting the glomerular permeability of anionic macromolecules such as albumin.
Treatment of isolated GBM with heparatinase to remove heparan sulfate proteoglycan
anionic side chains or profamine to effect charge neutralization had no effect on
the albumin sieving coefficient. Protamine decreased hydraulic conductivity. pH titration
to encompass the isoelectric point of GBM and albumin resulted in a modest increment
in albumin permeability. In contrast, when intact glomeruli were treated with either
heparatinase or protamine, albumin permeability doubled and protamine treatment of
glomeruli resulted in an even greater augmentation of albumin permeability. These
results suggest that, to the extent that in vitro GBM is similar to in vivo GBM, the
restriction in albumin permeability due to glomerular charge requires the presence
of glomerular cells.
Abbreviations:
GBM (glomerular basement membrane), HSPG (heparan sulfate proteoglycan)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
February 9,
1994
Received in revised form:
January 27,
1994
Received:
June 18,
1993
Footnotes
☆Supported by the American Heart Association, Minnesota Affiliate.
☆Presented in part at the 1990 Meetings of the American Society of Nephrology and the American Federation for Clinical Research.
Identification
Copyright
© 1994 Published by Elsevier Inc.
