Original Articles| Volume 135, ISSUE 3, P275-286, March 2000

Effects of dietary polyunsaturated fatty acid supplementation in early renal insufficiency in dogs


      Dietary supplementation with polyunsaturated fatty acids (PUFAs) alters the course of experimental kidney disease in dogs. In particular, supplementation with ω-6 PUFAs hastens the decline of kidney function, and ω-3 PUFAs are renoprotective. We investigated the early stages of renal insufficiency to determine whether PUFA supplementation altered the magnitude of hypercholesterolemia or glomerular hemodynamics. Two months after 11/12 nephrectomy, dogs were randomly divided into three groups of 6 animals each. Each group of dogs was then fed a low-fat basal diet supplemented with one of three sources of lipid to achieve a final concentration of 15% added fat. Fat sources were rich in ω-3 PUFAs (menhaden fish oil, group FO), ω-6 PUFAs (safflower oil, group SO), or saturated fatty acids (beef tallow, group C). Early in renal insufficiency, before significant kidney damage, group FO had a lower (P <.05) serum cholesterol concentration and tended to have a lower urinary prostaglandin E2 (PGE2) and thromboxane A2 (TxA2) excretion than group C. In contrast, group SO had a higher mean glomerular capillary pressure (P <.05) and more glomerular enlargement (P <.05) and tended to have higher eicosanoid excretion rates than group C. These differences in lipid metabolism, glomerular hypertension and hypertrophy, and urinary eicosanoid metabolism could explain, in part, the beneficial effects of ω-3 PUFAs and the detrimental effects of ω-6 PUFAs when administered on a long-term basis in this model of renal insufficiency. (J Lab Clin Med 2000;135:275-86)


      BUN (blood urea nitrogen), CCr (urinary clearance of exogenously administered creatinine), FF (renal filtration fraction), FO (menhaden fish oil), GBF (single glomerular blood flow), GFR (glomerular filtration rate (urinary clearance of inulin)), GPF (single glomerular plasma flow), Hct (hematocrit), HDL cholesterol (cholesterol contained in high-density lipoprotein particles), Kf (glomerular ultrafiltration coefficient), ΔP (glomerular transcapillary hydraulic pressure gradient), MAP (mean systemic arterial pressure), PGE2 (prostaglandin E2), PCP (peritubular capillary hydraulic pressure), PGC (glomerular capillary pressure), PSF (proximal tubular stop-flow pressure), πa (arterial colloid osmotic pressure), πe (glomerular efferent colloid osmotic pressure), PUFA (polyunsaturated fatty acid), RA (pre-glomerular vascular resistance), RBF (renal blood flow), RE (post-glomerular vascular resistance), RPF (renal plasma flow), RT (total renal vascular resistance), SCr (plasma concentration of creatinine), SNGFR (single nephron glomerular filtration rate), SO (safflower oil), TxB2 (thromboxane B2 (the stable metabolite of thromboxane A2))
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