Abstract
Uterine leiomyomas are the most common tumor of the genitourinary system in women
and are a major cause of morbidity. The molecular causes of the disease remain unclear.
In this study, we examined gene expression in leiomyomas and normal myometrium. RNA
was prepared and gene expression determined with the use of Affymetrix GeneChip U_95
arrays containing approximately 12,000 known genes and 48,000 expression sequence
tags. Several genes were found to be differentially expressed in these two sample
sets, and these genes were analyzed for their expression in a variety of other normal
and diseased tissues. Four genes—doublecortin, calpain 6, interleukin-17B,
and proteolipid protein 1—were found to be overexpressed in leiomyomas compared
with normal myometrium and eighteen other tissues. Sets of genes were identified whose
expression could be used to cluster samples with leiomyomas or normal myometrium with
the use of Eisen Cluster software. We conclude that differences in gene expression
can be detected between leiomyomas and normal myometrium and that these changes in
gene expression may yield clues to the pathophysiology of this common tumor.
Keywords:
cGMP (cyclic guanosine monophosphate), EGF (epithelial growth factor), IGF (insulin-like growth factor), IL (interleukin), MMP (matrix metalloproteinase), NGF (nerve growth factor), PDGF (platelet-derived growth factor), TCA (tricarboxylic acid cycle), TGF (transforming growth factor)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
November 26,
2002
Received in revised form:
October 28,
2002
Received:
August 8,
2002
Footnotes
Presented in part at the 93rd Annual Meeting of the American Society for Cancer Research, San Francisco, April 6-10, 2002 (Proc AACR 2002;43:466).
Identification
Copyright
© 2003 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
