Abstract
Mesenchymal stem cells (MSCs) have been cultured from many sources, including bone
marrow and liver. To further support our hypothesis that MSCs exist in most postnatal
tissues, we isolated a clonogenic, multipotent, rapidly proliferating population of
cells from a fetal pancreas and termed them “pancreas-derived mesenchymal
stem cells” (PMSCs). They withstood being passaged as many as 30 times without
sustaining significant structural changes. In this study, we showed that PMSCs are
positive for CD44, CD29, and CDI3 but negative for CD34 and HLA-DR and that they stained
with collagen I and III but not with von Willebrand factor antibody. During the log
phase of growth, PMSCs proliferated, doubling in population in about 30 hours. Cell-cycle
analysis showed that more than 90% of cells were in the G0 and G1 phases, whereas
a small subpopulation of cells were actively engaged in proliferation (S + G2 + M
= 3.55%). Under differentiation culture conditions, PMSCs differentiated into cells
of osteogenic, chondrogenic, and adipogenic lineages. These results demonstrate that
PMSCs can be isolated from human fetal pancreas by means of their adherent ability
and that they are capable of self-renewal, propagation, and multipotent differentiation.
Keywords:
dNTP (deoxyribornucleoside triphosphate), MSCs (mesenchymal stem cells), PMSCs (pancreas-derived mesenchymal stem cells), CFU-F (colony forming unit of fibroblast), FBS (fetal bovine serum)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
January 17,
2003
Received in revised form:
November 26,
2002
Received:
May 21,
2002
Footnotes
Supported by grants from the Pandeng Plan of the Ministry of Science and Technology of the People’s Republic of China (grant 95, special 10); the China Medical Board of New York, Inc: Stem Cell Biology, Engineering (grant 01-748); the National Natural Science Foundation of China (grant 30070284); the Beijing Ministry of Science and Technology (grant 2002-489); and the National Key Project for Basic Research of China (Grant 001CB5099). Dr Zhao is a Cheung Kong Scholar.
Identification
Copyright
© 2003 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
