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Abstract
In this paper, in addition to some points regarding the theory and practice of spectrophotometry,
we have presented: (1) an outline of a method for the preparation of solutions of
hematoporphyrin when present in urine, which affords a characteristic absorption zone
spectrophotometrically; (2) spectrophotometric curves of normal urine and of urine
containing hematoporphyrin; (3) methods for the calculation of the amount of hematoporphyrin
in 1000 c.c. of urine in terms of the equivalent number of cubic centimeters of blood
for each 1000 c.c. of urine; (4) applications of the laws of Lambert and Beer to the
spectrophotometric data obtained with dilution and at wave-length 555 millimicrons,
the point of maximal absorption of alcoholic solutions of redissolved acetic acid
precipitates of hematoporphyrin in urine, and (5) suggestions regarding a simplified
clinical procedure.
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References
- Eczema solare in a patient with hematoporphyrinuria.Arch. Dermat. & Syph. 1929; 10: 501-505
- A direct reading spectrophotometer.J. Optical Soc. Am. & Rev. Sc. Instruments. 1925; 2: 403-410
Article info
Publication history
Received:
April 24,
1929
Identification
Copyright
© 1929 Published by Elsevier Inc.
