Research Article| Volume 15, ISSUE 2, P162-169, November 1929

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The spectrophotometric determination of hematoporphyrin in urine

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      In this paper, in addition to some points regarding the theory and practice of spectrophotometry, we have presented: (1) an outline of a method for the preparation of solutions of hematoporphyrin when present in urine, which affords a characteristic absorption zone spectrophotometrically; (2) spectrophotometric curves of normal urine and of urine containing hematoporphyrin; (3) methods for the calculation of the amount of hematoporphyrin in 1000 c.c. of urine in terms of the equivalent number of cubic centimeters of blood for each 1000 c.c. of urine; (4) applications of the laws of Lambert and Beer to the spectrophotometric data obtained with dilution and at wave-length 555 millimicrons, the point of maximal absorption of alcoholic solutions of redissolved acetic acid precipitates of hematoporphyrin in urine, and (5) suggestions regarding a simplified clinical procedure.
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