Research Article| Volume 15, ISSUE 2, P170-171, November 1929

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Method for staining of polar bodies

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      A new method has been devised for the staining of metachromatic bodies with the following procedure: the smears are fixed on the flame as usual. The slides are covered for five minutes with solution I Gruebler's methylene blue 5 grams, 95 per cent alcohol 10 c.c., glacial acetic acid 5 c.c., distilled water q.s. 100 c.c. The stain is then removed with running water, and the slides are covered for one to two minutes with 1 per cent safranin or fuchsin 1:20. The slides are washed with water and dried. The metachromatic bodies appear deep blue; the bacterial bodies are distinctly red.
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