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Abstract
A new method has been devised for the staining of metachromatic bodies with the following
procedure: the smears are fixed on the flame as usual. The slides are covered for
five minutes with solution I Gruebler's methylene blue 5 grams, 95 per cent alcohol
10 c.c., glacial acetic acid 5 c.c., distilled water q.s. 100 c.c. The stain is then
removed with running water, and the slides are covered for one to two minutes with
1 per cent safranin or fuchsin 1:20. The slides are washed with water and dried. The
metachromatic bodies appear deep blue; the bacterial bodies are distinctly red.
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References
- Handb. d. Path. Mikroorg. 1912; 1 (Friedberger u. Reiter) (2nd ed.): 353
- Textbook of Bacteriology. W. B. Saunders Co.,, Philadelphia1927: 56
- IX ed. Textbook of Bacteriology. W. B. Saunders Co.,, Philadelphia1927: 71
- IX ed. Textbook of General Bacteriology. W. B. Saunders Co.,, Philadelphia1928: 56
Article info
Publication history
Received:
April 24,
1929
Footnotes
☆Department of Bacteriology, Pathology and Preventive Medicine, Loyola University School of Medicine.
Identification
Copyright
© 1929 Published by Elsevier Inc.
