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Research Article| Volume 26, ISSUE 2, P408-415, November 1940

Modified technique for prothrombin determination in blood

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      Abstract

      Due to the objections to the present tests in use for the determination of prothrombin in blood, a modified technique has been evolved which overcomes these defects and in addition has the essential factors of adaptability, simplicity, accuracy, and economy. A new thromboplastic agent which is stable and easily prepared has been made from sheep brain. The modified technique has been adequately tested clinically and has been found to be satisfactory.
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      References

        • Quick A.J.
        • Stanley-Brown M.
        • Bancroft F.W.
        Study of Coagulation Defect in Hemophilia and in Jaundice.
        Am. J. M. Sc. 1935; 190: 501
        • Bancroft F.W.
        • Kugelmass I.N.
        • Stanley-Brown M.
        Evaluation of Blood Clotting Factors in Surgical Diseases, With Special Reference to Thrombosis and Embolism and Certain Bleeding Conditions.
        Ann. Surg. 1929; 90: 161
        • Linton R.R.
        Relation of Blood Fibrin to Hemorrhagic Diathesis of Obstructive Jaundice.
        Ann. Surg. 1932; 96: 394
        • Scott F.H.
        • Chamberlain C.
        Calcium and Coagulation of Blood.
        in: Proc. Soc. Exper. Biol. & Med. 31. 1934: 1054
        • Ferguson J.H.
        Observations on Alterations of Blood Platelets as Factor in Coagulation of Blood.
        Am. J. Physiol. 1934; 108: 670
        • Ravdin I.S.
        • Riegel C.
        • Morrison J.L.
        Coagulation of Blood.
        Ann. Surg. 1930; 91: 801
        • Ravdin I.S.
        • Riegel C.
        • Morrison J.L.
        Coagulation of Blood.
        Ann. Surg. 1930; 91: 810
        • Wangensteen O.H.
        Hemorrhagic Diathesis of Obstructive Jaundice and Its Treatment.
        Ann. Surg. 1928; 88: 845
        • Nygaard K.K.
        Coagulability of Blood Plasma; Remarks on Technique of Its Determination.
        in: ed. 3. Proc. Staff Meet., Mayo Clin. 9. 1934: 151
        • Howell W.H.
        • Cekada E.B.
        Cause of Delayed Clotting of Hemophilic Blood.
        Am. J. Physiol. 1926; 78: 500
        • Bancroft F.W.
        • Quick A.J.
        • Stanley-Brown M.
        Anticoagulants of Blood.
        in: ed. 3. Proc. Soc. Exper. Biol. & Med. 32. 1934: 164
        • Warner E.D.
        • Brinkhous K.M.
        • Smith H.P.
        Plasma Prothrombin Levels in Various Vertebrates.
        Am. J. Physiol. 1939; 125: 296
        • Quick A.J.
        Nature of Bleeding in Jaundice.
        J. A. M. A. 1938; 110: 1658
        • Smith H.P.
        • Warner E.D.
        • Brinkhous K.M.
        Prothrombin Deficiency and Bleeding Tendency in Liver Injury (Chloroform Intoxication).
        J. Exper. Med. 1937; 66: 801
        • Warner E.D.
        • Brinkhous K.M.
        • Smith H.P.
        Quantitative Study on Blood Clotting; Prothrombin Fluctuations Under Experimental Conditions.
        Am. J. Physiol. 1936; 114: 667
        • Quick A.J.
        On Action of Heparin and Its Relation to Thromboplastin.
        Am. J. Physiol. 1936; 115: 317
        • Ferguson J.H.
        Quantitative Relationship of Calcium and Cephalin in Experimental Thrombin Formation.
        Am. J. Physiol. 1938; 123: 341
        • Ferguson J.H.
        Blood Calcium and Calcium Factor in Blood Coagulation.
        Physiol. Rev. 1936; 16: 640
        • Quick A.J.
        Coagulation Defects in Sweet Clover Disease and in Hemorrhagic Chick Disease of Dietary Origin; Consideration of Source of Prothrombin.
        Am. J. Physiol. 1937; 118: 260
        • Quick A.J.
        On Various Properties of Thromboplastin (Aqueous Tissue Extracts).
        Am. J. Physiol. 1936; 114: 282
        • Smith H.P.
        • Warner E.D.
        • Brinkhous K.M.
        Lung Extract in Blood Clotting.
        Am. J. Physiol. 1934; 107: 63
        • Snell A.M.
        Preliminary Report: Clinical and Experimental Conditions Associated With Deficiency of Prothrombin.
        in: Symposium on Hemorrhagic Diathesis in Cases of Jaundice: Its Relation to Vitamin K Deficiency. ed. 3. Proc. Staff Meet., Mayo Clin. 13. 1938: 65
        • Nygaard K.K.
        Coagulability of Blood Plasma.
        in: ed. 3. Proc. Staff Meet., Mayo Clin. 7. 1932: 544
        • Ziffren S.E.
        • Owen C.A.
        • Hoffman G.R.
        • Smith H.P.
        Control of Vitamin K Therapy. Compensatory Mechanisms at Low Prothrombin Levels.
        in: ed. 3. Proc. Soc. Exper. Biol. & Med. 40. 1939: 595
        • Owen C.A.
        • Hoffman G.R.
        • Ziffren S.E.
        • Smith H.P.
        Blood Coagulation During Infancy.
        in: ed. 3. Proc. Soc. Exper. Biol. & Med. 41. 1939: 181
        • Quick A.J.
        • Grossman A.M.
        Prothrombin Concentration in Newborn.
        in: ed. 3. Proc. Soc. Exper. Biol. & Med. 41. 1939: 227
        • Mertz E.T.
        • Seegers W.H.
        • Smith H.P.
        Inactivation of Prothrombin by Purified Thrombin Solutions.
        in: ed. 3. Proc. Soc. Exper. Biol. & Med. 41. 1939: 657