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Research Article| Volume 26, ISSUE 1, P174-183, October 1940

A discussion of electrophoresis with special reference to serum and allergens

  • H.A. Abramson
    Affiliations
    From the Electrophoresis Laboratory and the Department of Physiology, Columbia University, New York, N. Y., U.S.A.

    From the Medical Service of Dr. George Baehr and the Laboratories of the Mount Sinai Hospital, New York, N. Y., U.S.A.
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  • D.H. Moore
    Affiliations
    From the Electrophoresis Laboratory and the Department of Physiology, Columbia University, New York, N. Y., U.S.A.

    From the Medical Service of Dr. George Baehr and the Laboratories of the Mount Sinai Hospital, New York, N. Y., U.S.A.
    Search for articles by this author
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      Abstract

      The nature of the charging process of protein molecules leads to statistical values of the net charge. The large number of differences in the value of the net charge makes the electrophoretic technique desirable for purposes of separation of proteins from one another. This procedure has been greatly improved by the development of the Tiselius moving boundary technique. The application of the Tiselius technique to a study of the components of sera, to the properties of allergic antibodies, and to the fractionation of ragweed pollen extract is briefly outlined.
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