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Original article| Volume 132, ISSUE 1, P32-38, July 1998

Inhibition of 11β-hydroxysteroid dehydrogenase by bioflavonoids and their interaction with furosemide and gossypol

  • Junhua Guo
    Affiliations
    Departments of Pharmacology and Medicine, Cornell University Medical College, New York, New York, USA
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  • Marcus M. Reidenberg
    Correspondence
    Reprint requests: Marcus M. Reidenberg, MD, Department of Pharmacology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021.
    Affiliations
    Departments of Pharmacology and Medicine, Cornell University Medical College, New York, New York, USA
    Search for articles by this author
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      Abstract

      Inhibition of 11β-hydroxysteroid dehydrogenase (11β-OHSD) can cause a mineralocorticoid effect. This enzyme, in guinea pig renal cortex microsomes in vitro, is inhibited by quercetin, tea polyphenols, furosemide, and gossypol. The dietary polyphenols, quercetin and tea polyphenols, interact with furosemide to enhance its 11β-OHSD inhibitory effect. Tea polyphenols added to the effect of gossypol. We conclude that the diet contains inhibitors of 11β-OHSD that can alter the activity of this enzyme and may modify the degree of endogenous mineralocorticoid activity.

      Abbreviations:

      11β-OHSD (11β-Hydroxysteroid dehydrogenase), CI (combination index), IC50 (median inhibitory concentration), NAD (nicotinamide adenine dinucleotide), NADP (nicotinamide adenine dinucleotide phosphate)
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