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Abstract
In the hope of identifying agents of therapeutic value in immunoglobulin A nephropathy
(IgA-N), we tested crude methanol extracts of 15 Chinese herbs for their effect on
human mesangial cel proliferation in vitro. The results indicated that 7 out of the
15 crude extracts inhibited human mesangial cell proliferation activated by interleukin-1β
and interleukin-6. The extracts and their median inhibitory concentrations were as
follows (in μg/ml): Selaginella tamariscina (MLS-032), 56.0 ± 2.0; Ixeris chinensis (MLS-033), 62.7 ± 1.7; Polygonum hypoleucum Ohwi (MLS-034), 25.0 ± 1.5; Scutellaris rivularis (MLS-036), 39.6 ± 1.1; Condonacanthus paucifiorus (MLS-042),63.6 ± 2.6; Xanthium strumarium (MLS-043), 42.8 ± 1.3; Daemonoropus margaritae (MLS-044), 56.1 ± 1.9. These findings indicate that human mesangial cells were most
sensitive to MLS-034 treatment. These herbs also decreased interleukin-1β and tumor
necrosis factor-α production. Moreover, TNF-α mRNA expression was inhibited by MLS-034.
It is unlikely that cytotoxicity was involved, because no cell deaths were observable.
We hypothesize that the inhibitory mechanisms of these Chinese herbs may be related
to the impairments of gene expression and production of cytokines in human mesangial
cells. Plans are underway for the isolation of pure compounds from these Chinese herbs
and the elucidation of their mechanisms of action.
Abbreviations:
DMSO (dimethyl sulfoxide), EDTA (ethylenediaminetetraacetic acid), EIA (enzyme immunoassay), IgA-N (IgA nephropathy), IL-1 (interleukin-1), IL-6 (interleukin-6), MeOH (methanol), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide salt), SSCP (sodium chloride-sodium dihyrogen phosphate-disodium hydrogen phosphate), TNF-α (tumor necrosis factor-α)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
March 26,
1998
Received in revised form:
March 26,
1998
Received:
October 2,
1997
Footnotes
☆Supported in part by a grant-in-aid from the National Science Council, Republic of China (NSC84-2331-B-077-002).
Identification
Copyright
© 1998 Published by Elsevier Inc.
