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Abstract
Dietary supplementation with polyunsaturated fatty acids (PUFA) alters the course
of experimental renal disease in rats. However, chronic renal disease in other laboratory
animals and in human beings frequently responds differently to experimental manipulations.
We investigated the effects of variations in dietary PUFA composition on the chronic
course of induced renal disease in dogs. Two months after ![Math Eq]()
nephrectomy, dogs were randomly divided into three groups of seven animals each.
For the next 20 months, each group of dogs was fed a low-fat basal diet supplemented
with one of three sources of lipid to achieve a final concentration of 15% added fat.
Fat sources provided ω-3 PUFA (menhaden fish oil, group FO), ω-6 PUFA (safflower oil,
group SO), or saturated fatty acids (beef tallow, group BT). Throughout the dietary
trial, the magnitude of proteinuria and the plasma concentrations of creatinine, cholesterol,
and triglyceride were lower in group FO. The mean overall glomerular filtration rate
was 0.89 ± 0.18 ml/min per kilogram of body weight in group SO, a value that was significantly
less (p < 0.05) than the corresponding values for groups BT and FO (1.21 ± 0.18 and 1.43
± 0.20 ml/min/kg, respectively). Renal interstitial fibrosis also was significantly
elevated in group SO. The extents of mesangial matrix expansion, glomerulosclerosis,
and renal interstitial cellular infiltrate were similar in groups BT and SO, but lower
(p < 0.05) in group FO. We conclude that supplementation with ω-6 PUFA enhanced renal
injury; supplementation with ω-3 PUFA was renoprotective.
nephrectomy, dogs were randomly divided into three groups of seven animals each.
For the next 20 months, each group of dogs was fed a low-fat basal diet supplemented
with one of three sources of lipid to achieve a final concentration of 15% added fat.
Fat sources provided ω-3 PUFA (menhaden fish oil, group FO), ω-6 PUFA (safflower oil,
group SO), or saturated fatty acids (beef tallow, group BT). Throughout the dietary
trial, the magnitude of proteinuria and the plasma concentrations of creatinine, cholesterol,
and triglyceride were lower in group FO. The mean overall glomerular filtration rate
was 0.89 ± 0.18 ml/min per kilogram of body weight in group SO, a value that was significantly
less (p < 0.05) than the corresponding values for groups BT and FO (1.21 ± 0.18 and 1.43
± 0.20 ml/min/kg, respectively). Renal interstitial fibrosis also was significantly
elevated in group SO. The extents of mesangial matrix expansion, glomerulosclerosis,
and renal interstitial cellular infiltrate were similar in groups BT and SO, but lower
(p < 0.05) in group FO. We conclude that supplementation with ω-6 PUFA enhanced renal
injury; supplementation with ω-3 PUFA was renoprotective.Abbreviations:
BT (Beef tallow), BUN (blood urea nitrogen), CCr (urinary clearance of exogenously administered creatinine), FO (menhaden fish oil), GFR (glomerular filtration rate), HDLcholesterol (cholesterol contained in high-density lipoprotein particles), PAS (periodic acidSchiff), PUFA (polyunsaturated fatty acids), SCr (serum creatinine concentration), SO (safflower oil)To read this article in full you will need to make a payment
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renal ablation model.
reduction of renal mass.Article info
Publication history
Accepted:
December 5,
1997
Received in revised form:
November 3,
1997
Received:
March 13,
1997
Footnotes
☆Supported by the Morris Animal Foundation.
Identification
Copyright
© 1998 Published by Elsevier Inc.
