During the past few decades, zebrafish (Danio rerio) have been a workhorse for developmental biology and genetics. Concurrently, zebrafish
have proved highly accessible and effective for translational research by providing
a vertebrate animal model useful for gene discovery, disease modeling, chemical genetic
screening, and other medically relevant studies. Key resources such as an annotated
and complete genome sequence, and diverse tools for genetic manipulation continue
to spur broad use of zebrafish. Thus, the purpose of this introductory review is to
provide a window into the unique characteristics and diverse uses of zebrafish and
to highlight in particular the increasing relevance of zebrafish as a translational
animal model. This is accomplished by reviewing broad considerations of anatomic and
physiological conservation, approaches for disease modeling and creation, general
laboratory methods, genetic tools, genome conservation, and diverse opportunities
for functional validation. Additional commentary throughout the review also guides
the reader to the 4 new reviews found elsewhere in this special issue that showcase
the many unique ways the zebrafish is improving understanding of renal regeneration,
mitochondrial disease, dyslipidemia, and aging, for example. With many other possible
approaches and a rapidly increasing number of medically relevant reports, zebrafish
approaches enhance significantly the tools available for translational research and
are actively improving the understanding of human disease.
Abbreviations:
cDNA (complementary DNA), dpf (days post fertilization), ISH (in situ hybridization), GFP (green fluorescent protein), KD (knockdown), KO (knockout), MO (morpholino), mRNA (messenger RNA), ZFIN (the Zebrafish Model Organism Database), ZIRC (Zebrafish International Resource Center)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: November 04, 2013
Accepted:
October 28,
2013
Received in revised form:
October 24,
2013
Received:
October 7,
2013
Footnotes
Conflict of Interest: All authors have read the journal's policy on disclosure of potential conflicts of interest and have none to disclose.
Identification
Copyright
© 2014 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
