Intravenous immunoglobulin (IVIg), a therapeutic preparation containing pooled human
immunoglobulin (Ig) G, has been suggested to inhibit differentiation and maturation
of dendritic cells (DCs); however, controversies exist on this issue. We aimed to
reinvestigate the effects of IVIg on human DC maturation and cytokine production,
and to determine whether an artifactual determinant is involved in the observed effects.
Human monocyte-derived DCs or freshly isolated blood myeloid DCs were cultured in
the presence of IVIg in vitro, and the expression of maturation markers CD80, CD86, CD83, and Human Leukocyte Antigen-DR
were determined by flow cytometry, whereas production of interleukin (IL)-12 and IL-10
was measured by enzyme-linked immunosorbent assay, and T-cell stimulatory capacity
was determined in cocultures with allogeneic CD4+ T cells. Interestingly, we observed that IVIg did not inhibit, but instead stimulated,
spontaneous maturation and T-cell stimulatory ability of human DCs, while leaving
lipopolysaccharide-induced DC maturation and cytokine production unaffected. Strikingly,
prevention of IVIg binding to culture plate surface, or blocking of the activating
Fcγ receptor IIa on DC, abrogated the stimulatory effect of IVIg on costimulatory
molecule expression and on T-cell stimulatory capacity of DCs, suggesting that IVIg
activates DCs on IgG adsorption to the plastic surface. This study warrants for careful
study design when performing cell culture studies with IVIg to prevent artifactual
effects, and shows that IVIg does not modulate directly costimulatory molecule expression,
cytokine production, or allogeneic T-cell stimulatory capacity of human DCs.
Abbreviations:
DC (dendritic cell), FcγR (Fcγ receptor), FCS (fetal calf serum), HLA (Human Leukocyte Antigen), HSA (human serum albumin), IgG (immunoglobulin G), IL (interleukin), IVIg (intravenous immunoglobulin), LPS (lipopolysaccharide), mDC (myeloid dendritic cell), moDC (monocyte-derived dendritic cell)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: January 16, 2014
Accepted:
January 6,
2014
Received in revised form:
January 6,
2014
Received:
September 1,
2013
Identification
Copyright
© 2014 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
