Reported in vitro data implicated soluble CD40 ligand (sCD40L) in endothelial dysfunction
and angiogenesis. However, whether sCD40L could exert that influence in endothelial
dysfunction and angiogenesis after injury in acute myocardial infarction (AMI) patients
remains unclear. In the present study, we evaluated the association of sCD40L with
markers of platelet activation, endothelial, and vascular function during a recovery
period early after AMI. To achieve this goal, the time changes of soluble, platelet-bound,
and microparticle-bound CD40L levels over 1 month were assessed in AMI patients and
correlated with endothelial nitric oxide synthase (eNOS) polymorphisms, vascular endothelial
growth factor (VEGF) concentrations, and platelet expression of P-selectin (CD62P).
The association of soluble form, platelet-bound, and microparticle-bound CD40L with
CD62P expression on platelets, a marker of platelet activation, was also assessed
to evaluate the role of CD40L in the thrombosis, whereas the association with eNOS
and VEGF was to evaluate the role of CD40L in vascular dysfunction. This work shows
for the first time that time changes of sCD40L over 1 month after myocardial infarct
onset were associated with G894T eNOS polymorphism and with the VEGF concentrations,
but not to the platelet CD62P expression. These results indicate that, in terms of
AMI pathophysiology, the sCD40L cannot be consider just as being involved in thrombosis
and inflammation but also as having a relevant role in vascular and endothelial dysfunction.
Abbreviations:
AMI (acute myocardial infarction), APC (allophycocyanin), Asp (aspartate), cTNT (cardiac troponin), CAD (coronary artery disease), CRP (C-reactive protein), CK (creatine kinase), eNOS (endothelial nitric oxide synthase), ELISA (enzyme-linked immunosorbent assay), FITC (fluorescein isothiocyanate), FAU (fluorescence arbitrary units), Glu (glutamic acid), LME (linear mixed effects model), MPs (microparticles), NO (nitric oxide), NT-proBNP (N-terminal pro-brain natriuretic peptide), PCI (percutaneous coronary intervention), PBS (phosphate-buffered saline), PE (Phycoerythrin), CD62P (P-selectin), sCD40L (soluble CD40 ligand), SA (stable angina pectoris), VEGF (vascular endothelial growth factor)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: July 30, 2015
Accepted:
July 24,
2015
Received in revised form:
June 19,
2015
Received:
March 31,
2015
Identification
Copyright
© 2015 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
