The objective of this study was to develop a thermotriggered, polymer-based liposomal
drug carrier with an activatable magnetic resonance imaging (MRI) contrast property
for monitoring the release of substances and for localized tumor therapy. The multimodal
thermoactivatable polymer-grafted liposomes (MTPLs) were tested to investigate whether
the accumulation of MTPLs in colon-26 grafted tumors could be visualized in vivo using
MRI and optical imaging, whether MTPLs induce signal enhancement, reflecting the release
of their contents, after triggering by short-term heating (42.5°C for 10 minutes)
9 hours after MTPL administration (late-phase triggering), and whether MTPLs can provide
a sufficient antitumor effect. The imaging and therapeutic properties of MTPLs were
tested both in vitro and in vivo (BALB/c nude mice: heated group with MTPLs (n = 5),
nonheated group with MTPLs (n = 5), heated group with doxorubicin-free MTPLs (n = 5),
nonheated group with manganese-free MTPLs (n = 5), and kinetics observation group
(n = 3); N = 23). Through in vivo MRI and fluorescent imaging, the MTPLs were shown
to have significantly accumulated in the grafted colon-26 tumors 8 hours after administration.
Delayed thermotriggering (9 hours after administration) caused MR signal enhancement,
reflecting the release of their contents, after a short exposure to tolerable heat.
In addition, significant antitumor effects were observed after treatment. The proposed
polymer-based activatable MTPLs with a “delayed thermotrigger” provide a promising
technology for cancer theranostics that allows minimal adverse effects and rapid interactive
therapy.
Abbreviations:
2D (two-dimensional), DDS (drug delivery system), DPPC (dipalmitoylphosphatidylcholine), EPR (enhanced permeability and retention), FOV (field of view), MBE (manganese-bound relaxivity enhancement), Mn (manganese), MnSO4 (manganese sulfate), MRI (magnetic resonance imaging), MTPL (multimodal thermoactivatable polymer-grafted liposome), NA (number of acquisitions), PEG (polyethylene glycol), R1 (longitudinal relaxation rate), R2 (transverse relaxation rate), ST (slice thickness), T1 (longitudinal relaxation time), T2 (transverse relaxation time), TR (repetition time), TE (echo time), TPL (thermosensitive polymer-grafted liposome), TSL (temperature-sensitive liposomes)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: August 05, 2015
Accepted:
July 29,
2015
Received in revised form:
July 2,
2015
Received:
January 5,
2015
Identification
Copyright
© 2015 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
