There is an increasing interest in using long noncoding RNAs (lncRNAs) as biomarkers
in cancer. Predictive biomarkers in hepatocellular carcinoma (HCC) have great benefit
in the choice of therapeutic modality for HCC. The aim of this study is to assess
lncRNA–urothelial carcinoma associated-1 (lncRNA-UCA1) and WD repeat containing, antisense to TP53 (WRAP53) expression as novel noninvasive biomarkers for diagnosis of HCC in sera of HCC patients
compared with chronic hepatitis C virus (HCV) patients and healthy volunteers and
to analyze their relationship with respect to the clinicopathologic features. We retrieved
HCC characteristic lncRNAs, lncRNA-UCA1 and lncRNA-WRAP53, based on the microarray signature profiling (released by LncRNADisease database).
Quantitative reverse-transcriptase polymerase chain reaction assay (RT-qPCR) was then
used to evaluate the expression of selected lncRNAs in the serum of 160 participants.
Furthermore, in 20 of 82 HCC cases involved in the study, we examined the expression
of lncRNA-UCA1 and lncRNA-WRAP53 in 20 HCC tissues and adjacent nontumor tissues and analyzed its correlation with
the serum level of these lncRNAs. The prognostic significance of the investigated
parameters in HCC patients was explored. We found that lncRNA-UCA1 and lncRNA-WRAP53 were significantly higher in sera of HCC than those with chronic HCV infection or
healthy volunteers. Our data suggested that the increased expression of UCA1 and WRAP53 was associated with advanced clinical parameters in HCC. Of note, tissue levels of
the chosen lncRNAs strongly correlate with their sera level. The combination of both
lncRNAs with serum alpha fetoprotein resulted in improved sensitivity to 100%. The
median follow-up period was 21.5 months. LncRNA-WRAP53 was significant independent prognostic markers in relapse-free survival. LncRNA-UCA1 and lncRNA-WRAP53 upregulation may serve as novel serum biomarkers for HCC diagnosis and prognosis.
Abbreviations:
ALT (Alanine transaminase), AST (Aspartate transaminase), AFP (Alpha fetoprotein), CHC (Chronic HCV infection), HCC (Hepatocellular Carcinoma), lncRNA-UCA1 (Long noncoding RNA–Urothelial Carcinoma Associated-1), WRAP53 (WD repeat containing, antisense to TP53), qPCR (Quantitative reverse-transcriptase polymerase chain reaction assay)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: October 23, 2015
Accepted:
October 6,
2015
Received in revised form:
September 26,
2015
Received:
September 2,
2015
Identification
Copyright
© 2016 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
