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Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications

  • Seth Winfree
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind

    Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, Ind

    Indiana Center for Biological Microscopy, Indianapolis, Ind
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  • Michael J. Ferkowicz
    Affiliations
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Ind
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  • Pierre C. Dagher
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind

    Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, Ind

    Indianapolis Veterans Affairs Medical Center, Indianapolis, Ind
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  • Katherine J. Kelly
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind
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  • Michael T. Eadon
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind
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  • Timothy A. Sutton
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind
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  • Troy A. Markel
    Affiliations
    Department of Surgery, Indiana University School of Medicine, Indianapolis, Ind
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  • Mervin C. Yoder
    Affiliations
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Ind
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  • Kenneth W. Dunn
    Correspondence
    Reprint requests: Tarek M. El-Achkar (Ashkar), or Kenneth W. Dunn, Indiana University School of Medicine, Division of Nephrology, 950 W. Walnut, R2 E224, Indianapolis, IN 46202
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind

    Indiana Center for Biological Microscopy, Indianapolis, Ind
    Search for articles by this author
  • Tarek M. El-Achkar
    Correspondence
    Reprint requests: Tarek M. El-Achkar (Ashkar), or Kenneth W. Dunn, Indiana University School of Medicine, Division of Nephrology, 950 W. Walnut, R2 E224, Indianapolis, IN 46202
    Affiliations
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Ind

    Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, Ind

    Indianapolis Veterans Affairs Medical Center, Indianapolis, Ind
    Search for articles by this author
      Recent developments in automated optical sectioning microscope systems have enabled researchers to conduct high resolution, three-dimensional (3D) microscopy at the scale of millimeters in various types of tissues. This powerful technology allows the exploration of tissues at an unprecedented level of detail, while preserving the spatial context. By doing so, such technology will also enable researchers to explore cellular and molecular signatures within tissue and correlate with disease course. This will allow an improved understanding of pathophysiology and facilitate a precision medicine approach to assess the response to treatment. The ability to perform large-scale imaging in 3D cannot be realized without the widespread availability of accessible quantitative analysis. In this review, we will outline recent advances in large-scale 3D imaging and discuss the available methodologies to perform meaningful analysis and potential applications in translational research.

      Abbreviations:

      2D (2 dimensional), 3D (3 dimensional), LSM (Light sheet microscopy), IHCS (imaging high content screening), VTEA (Volumetric tissue exploration and analysis), 3DTC (3 dimensional tissue cytometry)
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