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3D Bioprinting of cardiac tissue and cardiac stem cell therapy

  • Matthew Alonzo
    Affiliations
    Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas
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  • Author Footnotes
    1 Denotes equal contribution.
    Shweta AnilKumar
    Footnotes
    1 Denotes equal contribution.
    Affiliations
    Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas
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  • Author Footnotes
    1 Denotes equal contribution.
    Brian Roman
    Footnotes
    1 Denotes equal contribution.
    Affiliations
    Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas
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  • Nishat Tasnim
    Affiliations
    Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas
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  • Binata Joddar
    Correspondence
    Reprint requests: Binata Joddar, Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, 500 W University Avenue, M201 J Engineering Building, El Paso, Texas.
    Affiliations
    Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas

    Border Biomedical Research Center, University of Texas at El Paso, El Paso, Texas
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  • Author Footnotes
    1 Denotes equal contribution.
Published:April 20, 2019DOI:https://doi.org/10.1016/j.trsl.2019.04.004
      Cardiovascular tissue engineering endeavors to repair or regenerate damaged or ineffective blood vessels, heart valves, and cardiac muscle. Current strategies that aim to accomplish such a feat include the differentiation of multipotent or pluripotent stem cells on appropriately designed biomaterial scaffolds that promote the development of mature and functional cardiac tissue. The advent of additive manufacturing 3D bioprinting technology further advances the field by allowing heterogenous cell types, biomaterials, and signaling factors to be deposited in precisely organized geometries similar to those found in their native counterparts. Bioprinting techniques to fabricate cardiac tissue in vitro include extrusion, inkjet, laser-assisted, and stereolithography with bioinks that are either synthetic or naturally-derived. The article further discusses the current practices for postfabrication conditioning of 3D engineered constructs for effective tissue development and stability, then concludes with prospective points of interest for engineering cardiac tissues in vitro. Cardiovascular three-dimensional bioprinting has the potential to be translated into the clinical setting and can further serve to model and understand biological principles that are at the root of cardiovascular disease in the laboratory.

      Abbreviations:

      BMSCs (bone marrow-derived mesenchymal stem cells), CFs (cardiac fibroblasts), CMs (cardiomyocytes), CMR (cardiac magnetic resonance), CNT (carbon nanotube), CVD (cardiovascular disease), CSCs (cardiac stem cells), CT (computer tomography), EBB (extrusion-based bioprinting), ECs (endothelial cells), ECM (extracellular matrix), GelM2A (gelatin methacrylate), human iPSCs (human induced pluripotent stem cells), HUVECs (human umbilical vein endothelial cells), LAB (laser-assisted bioprinting), LIFT (laser induced forward transfer), MSCs (mesenchymal stem cells), PEGDA (poly(ethylene glycol) dimethacrylate), RGD (arginine-glycine-aspartate)
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