The ability to generate human retinas in vitro from pluripotent stem cells opened unprecedented opportunities for basic science
and for the development of therapeutic approaches for retinal degenerative diseases.
Retinal organoid models not only mimic the histoarchitecture and cellular composition
of the native retina, but they can achieve a remarkable level of maturation that allows
them to respond to light stimulation. However, studies evaluating the nature, magnitude,
and properties of light-evoked responsivity from each cell type, in each retinal organoid
layer, have been sparse. In this review we discuss the current understanding of retinal
organoid function, the technologies used for functional assessment in human retinal
organoids, and the challenges and opportunities that lie ahead.
Abbreviations:
CNG (cyclic nucleotide-gated channel), HCN (hyperpolarization-activated cyclic nucleotide-gated channel), hESC (human embryonic stem cell), hiPSC (human induced pluripotent stem cell), hPSC (human pluripotent stem cell), IHC (immunohistochemistry), MEA (multi electrode array), NR (neural retina), RPE (retinal pigmented epithelium), TEM (transmission electron microscopy)To read this article in full you will need to make a payment
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Article Info
Publication History
Published online: June 08, 2022
Accepted:
June 1,
2022
Received in revised form:
May 31,
2022
Received:
April 8,
2022
Identification
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© 2022 Elsevier Inc. All rights reserved.
